求请教,双荧光素酶实验结果图怎么解读呢

楼主  收藏   举报   帖子创建时间:  2018-03-15 15:46 回复:2 关注量:397

想请教NC,LNA都代表什么意思????? mir-34a表达的检测,,那么对应的mir-nc,mir-lna 和mir-34a等关系都是什么,个代表什么呀,,荧光素酶,强度越高就是结合的越多吗? 

  • tf3319435 2018-03-15 15:48
    #1

  • mpark 2018-03-15 23:31
    #2

    LNA: locked nucleic acid. Try to use the link:

    https://en.wikipedia.org/wiki/Locked_nucleic_acid

    for more details.

    LNA is nothing to do luciferase assay. miR-LNA indicates micRNA is synthesized with LNA modification. In the figure, C is a result to normalize different synthesized microRNA or negative control (NC) exprerssion in testing cell lines, such as BT549, etc. D, a kind of result of luciferase assay upon different micRNA or NC transfection.