哪一个条带可能是全基因组DNA?

楼主  收藏   举报   帖子创建时间:  2018-01-25 19:33 回复:4 关注量:382

我们用TriZol从细胞里面提出了DNA,然后配了0.5%的琼脂糖胶,用TAE配的,电压120V,30min,跑出来的结果如图:想知道哪一条是跑出来的DNA,这样的结果能用来做DNA甲基化芯片吗?谢谢大家!

 

  • mpark 2018-01-26 00:58
    #1

    Please label the gel picture.

  • 雪丹5229 2018-03-15 16:51
    #2

    我们觉得sample那条带的下方亮亮的条带是内皮细胞的基因组,不知道各位大神的看法?它比较弥散,是因为什么原因呢?这样子的DNA可以拿来跑甲基化芯片吗?


  • mpark 2018-03-15 23:12
    #3

    In sample lane the most fast-running bands could be RNA, the very slow running bands at the top (larger than 16 kb) could be genomic DNA, the signals staggered in the well, junk. The smear, could be from junk or from degraded genomic DNA contaminated in sample preparation.


  • 雪丹5229 2018-03-16 09:44
    #4

    mpark

    In sample lane the most fast-running bands could be RNA, the very slow running bands at the top (larger than 16 kb) could be genomic DNA, the signals staggered in the well, junk. The smear, could be from junk or from degraded genomic DNA contaminated in sample preparation.


    Thanks very much !