Collagenase胶原蛋白酶来源于溶组织梭菌C7657 1G SIGMA原装_蛋白纯化_蛋白纯化_生化试剂库_商城

货号：	C7657
保存条件：	-20℃
CAS号：	9001-12-1
供应商：	sigma
数量：	1
英文名：	Collagenase from Clostridium histolyticum
规格：	1G

货号：C7657

名称：Collagenase from Clostridium histolyticum

中文名：胶原蛋白酶来源于溶组织梭菌

CAS号：9001-12-1

分子质量: 647

储存条件: -20 ℃

规格：1G

厂家：SIGMA

溶组织梭菌的培养滤液中获得此胶原酶

产品名称

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca²⁺) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of the reaction varies from 15 minutes to several hours to yield a satisfactory efficiency of cell dissociation without causing too much cell death. Krebs Ringer buffer with calcium and BSA is preffered and Zn²⁺ is required for activity. This enzyme is tested for the release of hepatocytes at approximately 1 mg/mL concentration, in a total volume of 100 mL for each rat liver.

Preparation Note

A stock solution may be prepared by dissolving 0.05-0.1 mg/mL of collagenase in 50 mM TES buffer containing 0.36 mM calcium chloride (TESCA), pH 7.4, at 37 °C.

Quality

也具有梭菌蛋白酶、非特异性中性蛋白酶和胰蛋白酶活性。

Unit Definition

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Application

Clostridiopeptidase A has been used to study the avoidance of the progression of necrotic tissue in neonates as well as to evaluate the effect of clostridiopeptidase A collagenase (CAC) in a rat model of postoperative peritoneal adhesion. Clostridiopeptidase A has also been used in a study to describe a quantitative method to assess small amounts of collagen based on MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass spectrometry (MS) subsequent to digestion of collagen.

This product has been used in the isolation of pancreatic ductal adenocarcinoma (PDAC) cell lines and the tissue culture of pancreatic fibroblasts.

性质

Related Categories	3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Dissociation, 更多...
solubility	TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble0.05-0.1 mg/mL at 37 °C
suitability	release of physiologically active rat pancreatic islets tested
Featured Industry	Diagnostic Assay Manufacturing
storage temp.	−20°C