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| 货号： | 345810-5GMCN |
G418 also known as Geneticin® is an aminoglycoside antibiotic related to Gentamicin. Used as a selective agent in transfection of eukaryotic cells. Has highest potency ≥730 µg/mg and purity ≥98%.
| Description | |
|---|---|
| Overview | Aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterialneor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418. |
| Also available as a sterile-filtered aqueous solution (Cat. No. 345812), a sterile filtered solution in 100 mM HEPES (Cat. No. 508940), and a sterile-filtered solution PBS (Cat. No.509227). | |
| Catalogue Number | 345810 |
| Brand Family | Calbiochem® |
| Synonyms | NMD Inhibitor I, G418, G 418 |
| References | |
|---|---|
| References | Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189. Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245. Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY. Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46. Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8. Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166. Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356. Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031. Jimenez, A., and Davies, J. 1980. Nature 287, 869. |
| Product Information | |
|---|---|
| CAS number | 108321-42-2 |
| Form | White powder |
| Hill Formula | C�6�0�6�8H�6�2�6�8N�6�2O�6�9�6�8 · 2H�6�0SO�6�2 |
| Chemical formula | C�6�0�6�8H�6�2�6�8N�6�2O�6�9�6�8 · 2H�6�0SO�6�2 |
| Structure formula Image | |
| Biological Information | |
|---|---|
| Biological activity | Assay: ≥730 µg/mg |
| Purity | ≥98% by TLC |
| Physicochemical Information | |
|---|---|
| Contaminants | Ammonia: ≤1.0%; heavy metals: ≤100 ppm |
| Safety Information according to GHS | |
|---|---|
| RTECS | CB9378500 |
| Storage and Shipping Information | |
|---|---|
| Ship Code | Ambient Temperature Only |
| Toxicity | Standard Handling |
| Storage | 29 |
| Do not freeze | Ok to freeze |
| Special Instructions | Following reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C. |
温馨提示：不可用于临床治疗。
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