Caspase-1抑制剂药物筛选试剂盒

点击图片查看原图
单价: 6000.00
品牌: Biovision
销量: 累计出售 0
评价: 已有 0 条评价
人气: 已有 26 人关注
更新: 2021-03-15
数量: 减少 增加份 库存999份
立即购买   加入购物车
看了又看 更多>
 
 
货号: K151-100
英文名: Caspase-1 Inhibitor Drug Screening Kit
保存条件: -20℃
供应商: 上海研卉生物科技有限公司
数量: 大量
规格: 100次分析

Kit Summary:
• Detection method- FFluorescence (Ex/Em 400/505)
• Sample type- Cell samples treated with Caspase inhibitor drugs
• Species reactivity- Mammalian
• Kit size- 100 assays
• Applications- Effective means for screening caspase inhibitors using fluorometric methods.

Features & Benefits:
• Simple one-step procedure; takes only 1-1.5 hours
• Fast and convenient
• Inhibition of caspase-1 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.

Kit components:
• 2X Reaction Buffer
• Caspase Substrate YVAD-AFC (1 mM)
• DTT (1 M)
• Active Caspase-1 (Lyophilized)
• Caspase Inhibitor, Z-VAD-FMK (2 mM)

Description:
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase-1 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate YVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-1 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-1 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

温馨提示:不可用于临床治疗。