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份 库存999份
| 货号： | K725-100 |
| 保存条件： | -20度 |
| 供应商： | 北京博迈斯 |
| 英文名： | Phospholipase D (PLD) Activity Colorimetric Assay Kit |
| 规格： | 100 assays |
Assay Kit Name:
Phospholipase D (PLD) Activity Colorimetric Assay Kit
Kit Summary:
• Detection method - Absorbance (OD 570 nm)
• Species reactivity - N/A
• Applications - Measurement of PLD activity in various tissues/cells
- Analyzing intracellular signaling
- Screening of PLD inhibitors
Sample Type:
• Animal tissues: liver, brain, kidney, etc.
• Cell culture: Adherent or suspension cells
Features & Benefits:
• Simple, rapid & convenient
• The assay can detect phospholipase D (PLD) Activity less than 1 mU/ml in various sample types.
Kit Components:
• PLD Assay Buffer
• PLD Probe (in DMSO)
• PLD Substrate
• PLD Enzyme Mix (Lyophilized)
• Choline Standard (Lyophilized)
• PLD Positive Control (Lyophilized)
Description:
Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders. BioVision’s Phospholipase D Activity Assay Kit provides a simple and sensitive method of measuring PLD activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline from Phosphatidylcholine. The free choline is then oxidized by PLD enzyme mix to generate an intermediate which reacts with PLD Probe to generate color (OD 570 nm). This high-throughput adaptable assay kit can detect PLD activity as low as 1.0 mU/ml in a variety of samples.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.
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