微粒体（Microsome）分离试剂盒_细胞因子、趋化因子和生长因子_细胞培养_生化试剂库_商城

货号：	K249-50
供应商：	北京博迈斯
英文名：	Microsome Isolation Kit
保存条件：	-20度
规格：	50 assays

微粒体（Microsome）在细胞生物学中定义为从内质网的碎片所得到的小型囊泡。多数情况下，微粒体是指在细胞匀浆和差速离心过程中获得的由破碎的内质网自我融合形成的近似球形的膜囊泡状结构，这些小囊泡的直径大约100 nm左右, 是异质性的集合体，它包含内质网膜和核糖体两种基本成分。在体外实验中，必须将肝脏或其他组织磨碎（均质化）之后，才能得到微粒体。微粒体含有细胞色素P450 (CYP)氧化酶，与氧化代谢有关，具有蛋白质合成、蛋白质糖基化和脂类合成等内质网的基本功能。科学家们常用微粒体来模拟和探索内质网的功能，以及药物代谢（筛选）方面的研究。

图一，微粒体结构示意图
常见的提取方法主要是靠超速离心法或者蔗糖密度梯度离心法，不仅费时费力，而且提取效果一般。为此，作为全球生命科学公司的领导者Biovision公司，为解决上述困境，精心研发而推出市场上唯一的微粒体分离提取试剂盒（K249-50）
微粒体（Microsome）分离试剂盒有以下特点：
方法简便：专利配方和方案，可无需超速离心和蔗糖密度梯度离心

. 快捷：1小时内提取具有活性的微粒体

. 适用于：哺乳动物腺体，软组织（肝，脾脏，肺…），类似于HepG2的细胞系

图2：微粒体分离提取效果图

Assay Kit Name:
Microsome Isolation Kit

Kit Summary:
• Detection method -
• Applications - Convenient and fast isolation of microsomal fraction from animal tissues
- Assessment of CYP-mediated drug metabolism and xenobiotic biotransformation
- Protein profiling of microsomal membrane proteins by SDS-PAGE and Western blot

Sample Type:
• Mammalian glands and soft tissues such as liver, spleen, lungs etc.
• Cultured eukaryotic cell lines such as HepG2 human hepatic carcinoma cells

Features & Benefits:
• Simple & convenient protocol
• The kit provides unique formulations of ready-to-use buffers and reagents to isolate microsomes without the need for ultracentrifugation or sucrose gradient fractionation

Kit Components:
• Homogenization Buffer
• Storage Buffer
• Protease Inhibitor Cocktail

Description:
Microsomes are spherical vesicle-like structures formed from membrane fragments following homogenization and fractionation of eukaryotic cells. The microsomal subcellular fraction is prepared by differential centrifugation and consists primarily of membranes derived from the endoplasmic reticulum (ER) and Golgi apparatus. Microsomes isolated from liver tissue are used extensively in pharmaceutical development, toxicology and environmental science to study the metabolism of drugs, organic pollutants and other xenobiotic compounds by the cytochrome P450 monooxidase (CYP) enzyme superfamily. Microsomal preparations are an affordable and convenient in vitro system for assessing Phase I biotransformation reactions, as they contain all of the xenobiotic-metabolizing CYP isozymes and the membrane-bound flavoenzymes (such as NADPH P450-Reductase and cytochrome b5) required for function of the multicomponent P450 enzyme system. BioVision’s Microsome Isolation Kit enables preparation of active microsomes in about one hour, without the need for ultracentrifugation or sucrose gradient fractionation. The kit contains sufficient reagents for 50 isolation procedures, yielding microsomes from roughly 25 grams of tissue or cultured cells.

Storage Conditions:
-20°C

温馨提示：不可用于临床治疗。

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微粒体（Microsome）分离试剂盒

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