SD-008/SN-009 Minute™ 总蛋白提取试剂盒(植物组织)

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单价: 2875.00
品牌: Invent
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更新: 2021-05-13
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货号: SD-008/SN-009
英文名: Minute TM Total Protein Extraction Kit for Plant Tissues
保存条件: RT
规格: 50tests

MinuteTM 植物组织总蛋白提取试剂盒

目录号:SD-008/SN-009

 

 

描述  

      植物组织总蛋白提取试剂盒,由优化细胞裂解液和0.6 ml离心管柱组成,能够迅速从植物组织(叶片,种子,柔软的干和根等)中提取总蛋白。试剂盒同时提供天然和变性两种不同的裂解液,可供用户选择。离心管柱技术提取蛋白质,可从20-200mg植物组织中中提取总蛋白质,操作时间5-8分钟,得率可达2-8mg/ml。

 

应用  

 

提取的蛋白质样品可以应用于SDS-PAGE, immunoblottings, ELISA, IP, ELISA,蛋白质分析和小规模蛋白层析纯化。

 

 

Figure 1. Plant protein profiles extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, snow pea; lane 2, corn kernel; 3, broccoli leaves; 4, spinach leaves and 5, chili pepper leaves.

 

 

操作步骤

操作流程图

 Protocol  

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产品订购信息  
Minute TM Total Protein Extraction Kit for Plant Tissues (50 Tests)  SD-008/SN-009 2875.00
Minute TM Total Protein Extraction Kit for Plant Tissues (4Tests)   SD-008/SN-009 290.00
参考文献  

1. Crystal R. et al. (2013). Expression profiling of four defense-related buffalograss transcripts in response to chinch bug (hemiptera: Blissidae) feeding. Journal of Economic Entomology. 106 (6):2568-2576.
2. Weber, C. F. (2017). Microgreen Farming and Nutrition: A Discovery-Based Laboratory Module to Cultivate Biological and Information Literacy in Undergraduates. The American Biology Teacher, 79(5), 375-386.
3. Abdulazeez, M., Abubakar, S. M., & Mu’azzam, J. M. (2017). Isolation and Characterization of a Potential Angiotensin-Converting Enzyme Inhibitory Peptide from the Leaves of Leptadenia hastata (Asclepiadaceae). Malaysian Journal of Applied Sciences, 2(1), 35-47. 

温馨提示:不可用于临床治疗。