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份 库存999份
| 货号： | CQMT |
| 供应商： | 北京绿源伯德生物科技有限公司 |
| 数量： | 100 |
| 英文名： | CellQuanti-MTT™ Cell Viability Assay Kit |
| 保质期： | 12个月 |
| 保存条件： | -20°C |
| 规格： | 500T |
Application
• Homogeneous assay for cell viability, proliferation, cytotoxcity and evaluation of anticancer agents.
Key Features
• Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).
• Sensitive and accurate. As low as 950 cells can be accurately quantified.
• Fast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day.
• Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
• Robust and amenable to HTS. Z’ factors of 0.5 and above are observed. Can be readily automated with HTS liquid handling systems.
Method
• OD570nm
Samples
• Cell culture
Species
• All
Procedure
• 5 hrs
Size
• 500 tests
Detection Limit
• 950 cells
美国进口细胞活性(MTT)测试盒|博世生物现货
Shelf Life
• 12 months
More Details
• The study of cell proliferation and cell viability requires the accurate quantification of the number of viable cells in a cell culture. Therefore, assays for calculating cell viability are necessary for optimizing cell culture conditions, evaluating cell growth factors and nutrients, discovering novel antibiotics and anti-cancer drugs, evaluating toxic effects of environmental pollutants and cell mediated toxicity and studying programmed cell death (apoptosis). The CellQuanti-MTT™ assay kit provides a convenient, sensitive, quantitative and reliable assay for determining the number of viable cells in a given culture. This homogeneous colorimetric assay is based on the conversion of a tetrazolium salt MTT, a pale yellow substrate, to formazan, a purple dye. This cellular reduction reaction involves the pyridine nucleotide cofactors NADH/NADPH and is only catalyzed by living cells. The formazan product has a low aqueous solubility and is present as purple crystals. Dissolving the resulting formazan with a solubilization buffer permits the convenient quantification of product formation. The intensity of the product color, measured at 550 - 620 nm, is directly proportional to the number of living cells in the culture. Reagents in the kit have been carefully formulated and optimized for sensitivity, assay robustness and automation.
博世生物技术有限公司(BioAssay Systems) 是一家位于美国北加州湾区的高新生物技术公司。博世生物以研发、生产和销售高品质的生物检测制剂为主；公司产品种类齐全，主要用于科学研究和生物医药开发；客户涵盖药物研发公司、生物技术公司、各大专院校、医院、环境和食品卫生部门等。客户使用我们的产品在重要学术刊物发表论文。迄今产品远销北美、南美洲、亚太地区、欧洲、非洲各地，及中国的北京、上海、天津、厦门、成都等大中城市。
北京绿源伯德生物科技有限公司欢迎咨询订购美国博世生物产品，
电话：15300145331 QQ：1961386367。咨询提供中文版说明书资料。
博世生物提供优质、快速(如2分钟)、方便的生化检测试剂盒，广泛适用于人和动物等生物样品、食品和环境监测样品中相关指标的检测。博世专注于无害、无放射性的检测技术(如吸收光、荧光、生物化学发光法等)，安全可靠，适合各类仪器(分光光度仪、微孔读数仪等）。产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征。检测步骤简便，用户不需花额外的时间来作调适测试。
美国进口细胞活性(MTT)测试盒|博世生物现货
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温馨提示：不可用于临床治疗。
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