Mouse Anti-RFP-Tag antibody
产品编号 | hz-33011M |
英文名称 | RFP-Tag |
中文名称 | 红色荧光蛋白(标签)单克隆抗体 |
别 名 | GFP like chromoprotein; red fluorescent protein; Red fluorescent protein eqFP611; RFP; RFPTag; RFP Tag. |
产品类型 | 标签抗体 |
研究领域 | |
抗体来源 | Mouse |
克隆类型 | Monoclonal |
克 隆 号 | 7A5 |
交叉反应 | Red fluorescent protein |
产品应用 | ELISA=1:1000-5000 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | Recombinant Red fluorescent protein: |
亚 型 | IgG |
纯化方法 | affinity purified by Protein G |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Red Fluorescent Protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm. Function: Pigment protein. Post-translational modifications: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), oxidation of Tyr-(N+1) to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen. SWISS: N/A Gene ID: N/A Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |