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Mouse Anti-GAPDH(3E12)-Loading Control antibody
| 产品编号 | hz-0978M |
| 英文名称 | GAPDH(3E12)-Loading Control |
| 中文名称 | 3-***酸甘油醛脱氢酶单克隆抗体(内参抗体) |
| 别 名 | 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10. |
| 产品类型 | 内参抗体 |
| 研究领域 | 肿瘤 免疫学 神经生物学 信号转导 通道蛋白 新陈代谢 Alzheimers |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 3E12 |
| 交叉反应 | Human, Mouse, Rat, Rabbit, |
| 产品应用 | WB=1:500-5000 ELISA=1:1000-5000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 38kDa |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Full length GAPDH protein purified from rabbit muscle: |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Loading Control |
| 产品图片 |  Sample: Placenta (Mouse) Lysate at 40 ug 293T Cell Lysate at 40 ug Primary: Anti-GAPDH(bsm-0978M)at 1/1000 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 38kD Observed band size: 38kD Sample: Bladder(Rat)Lysate at 40 ug Primary: Anti-GAPDH(bsm-0978M)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 38kD Observed band size: 38kD Sample:Liver(Mouse)Lysate at 40 ug Primary: Anti-GAPDH(bsm-0978M)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 38kD Observed band size: 38kD Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-GAPDH(3E12)-Loading Control (bsm-0978M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD Paraformaldehyde-fixed, paraffin embedded (Rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH(3E12)-Loading Control) Polyclonal Antibody, Unconjugated (bsm-0978M) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB stainin |
