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Anti-RBM35A/ESRP1抗体
| 产品编号 | YB-19771R |
| 英文名称 | RBM35A/ESRP1 |
| 中文名称 | RNA结合蛋白35A抗体 |
| 别 名 | Epithelial splicing regulatory protein 1; Esrp1; ESRP1_HUMAN; FLJ20171; RBM35A; RMB35A; RNA binding motif protein 35A; RNA-binding motif protein 35A; RNA-binding protein 35A. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 结合蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Rabbit, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 76kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RBM35A:151-250/681 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: ESPR1 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 [PubMed 19285943]).[supplied by OMIM, Aug 2009] Function: mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. Subcellular Location: Nucleus. Tissue Specificity: Epithelial cell-specific. Similarity: Belongs to the ESRP family. Contains 3 RRM (RNA recognition motif) domains. SWISS: Q6NXG1 Gene ID: 54845 Database links: Entrez Gene: 538640 Cow Entrez Gene: 487054 Dog Entrez Gene: 100055214 Horse Entrez Gene: 54845 Human Entrez Gene: 207920 Mouse Entrez Gene: 100156237 Pig Entrez Gene: 500409 Rat Omim: 612959 Human SwissProt: Q6NXG1 Human SwissProt: Q3US41 Mouse SwissProt: B2RYD2 Rat Unigene: 487471 Human Unigene: 387719 Mouse Unigene: 161971 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBM35A) Polyclonal Antibody, Unconjugated (bs-19771R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
