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| • | Pellet-Free™. No conventional cell-pelleting or resuspension steps required. |
| • | The fastest, simplest procedure for purifying the highest quality endotoxin-free plasmid DNA. |
| • | Innovative colored buffers for error-free visual identification of complete bacterial cell lysis and neutralization. |
| • | Format: Spin Column |
| • | Plasmid DNA Yield: Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. |
| • | Plasmid DNA Size: Up to 25 kb. |
| • | Recovery Volume: ≥30 µl |
| • | DNA Purity: Plasmid DNA which is well suited for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical A(260/280) index is ≥1.8. |
| • | Procedure: Performed at room temperature (15-30°C) using a centrifuge and/or a vacuum manifold. |
The Zyppy™ Plasmid Miniprep Kit features a Pellet-Free™ modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, then purify using our Fast-Spin™ column technology (alternatively, the samples may be processed by the classical centrifugation method). Additionally, the innovative colored buffers included in the kit permit error-free visualization identification of complete bacterial cell lysis and neutralization.
Our Zyppy™ Plasmid Miniprep Kit is the fastest and simplest method available to efficiently separate plasmid DNA from E. coli. The plasmid DNA is of the highest quality, is endotoxin-free, and is well suited for use in bacterial transformation, restriction endonuclease digestion (below), DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications. An overview of the purification procedure is shown to the upper right.
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ORDERING INFORMATION
Product Catalog No. Size Price
Zyppy™ Plasmid Miniprep Kit D4036 50 Preps
D4019 100 Preps
D4020 400 Preps
Free Sample D4036s 10 Preps Free
