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Anti-PBXIP1抗体
| 产品编号 | YB-19901R |
| 英文名称 | PBXIP1 |
| 中文名称 | 造血相互作用蛋白PBX抗体 |
| 别 名 | 4732463H20Rik; Hematopoietic PBX interacting protein; HPIP; MGC38361; Pre B cell leukemia homeobox interacting protein 1; RP11-307C12.2. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 染色质和核信号 干细胞 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Cow, Horse, Sheep, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 81kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PBXIP1:631-731/731 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: PBXIP1 is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. It inhibits the binding of PBX1-HOX complex to DNA and blocks the transcriptional activity of E2A-PBX1. PBXIP1 also tethers estrogen receptor-alpha (ESR1) to microtubules and allows them to influence estrogen receptors-alpha signaling. Function: Regulator of pre-B-cell leukemia transcription factors (BPXs) function. Inhibits the binding of PBX1-HOX complex to DNA and blocks the transcriptional activity of E2A-PBX1. Tethers estrogen receptor-alpha (ESR1) to microtubules and allows them to influence estrogen receptors-alpha signaling.3 Publications Manually curated information for which there is published experimental evidence. More… Manual assertion based on experiment ini Ref.1"Functional cloning and characterization of a novel nonhomeodomain protein that inhibits the binding of PBX1-HOX complexes to DNA." Abramovich C., Shen W.-F., Pineault N., Imren S., Montpetit B., Largman C., Humphries R.K. J. Biol. Chem. 275:26172-26177(2000) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH PBX1; PBX2 AND PBX3, VARIANT ASP-357. Ref.6"Functional characterization of multiple domains involved in the subcellular localization of the hematopoietic Pbx interacting protein (HPIP)." Abramovich C., Chavez E.A., Lansdorp P.M., Humphries R.K. Oncogene 21:6766-6771(2002) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, CHARACTERIZATION, SUBCELLULAR LOCATION. Ref.8"An inherent role of microtubule network in the action of nuclear receptor." Manavathi B., Acconcia F., Rayala S.K., Kumar R. Proc. Natl. Acad. Sci. U.S.A. 103:15981-15986(2006) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ESR1, MUTAGENESIS OF 615-LEU--LEU-619. Subunit: Interacts with TEX11 (By similarity). Interacts with ESR1, PBX1, PBX2 and PBX3. Subcellular Location: Cytoplasm, cytoskeleton. Nucleus. Note=Shuttles between the nucleus and the cytosol. Mainly localized in the cytoplasm, associated with microtubules. Detected in small amounts in the nucleus. Tissue Specificity: Expressed in early hematopoietic precursors. SWISS: Q96AQ6 Gene ID: 57326 Database links:Entrez Gene: 57326 Human Entrez Gene: 229534 Mouse Entrez Gene: 310644 Rat SwissProt: Q96AQ6 Human SwissProt: Q3TVI8 Mouse SwissProt: A2VD12 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PBXIP1) Polyclonal Antibody, Unconjugated (bs-19901R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining |
