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Description
GenJet™ DNA In Vitro Tranfection Reagent for BHK-21 cell is pre-optimized for transfecting BHK-21 cells. BHK-21 cells is one of the most commonly used cells for vaccine production. When used for this application, usually a cell strain adapted to grow in suspension is used. Refer to the following optimal transfection conditions for maximal transfection efficiency on BHK-21 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) | 80% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, 5 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter) 85% |
Storage ConditionStore at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet.pdf
