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Description:GenMute™ siRNA Transfection Reagent for C2C12 Cell is pre-optimized for transfecting siRNA to C2C12 cells. C2C12 cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush injury. These cells were shown to be capable of differentiation. C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins and the androhen receptor (AR).Size & content:- GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles siRNA or miRNA mimics in 24-well plate- GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solutionRefer to the following optimal transfection conditions for maximal silencing on C2C12 cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Cell culture medium Confluency of cell on the day of transfection Optimal siRNA concentration Diluent for siRNA and GenMute™ Reagent Incubation Time to Form GenMute™/siRNA Complex Maximal Silencing Efficiency Transfection Results: Reporter Gene Silencing Efficiency (% ) | DMEM with 4.5 g/L glucose, 10% FBS ~50% 40 nM 1xGenMute™ Transfection Buffer ~15 minutes at RT 48 hours Renilla Luciferase 80% |
Storage:Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Data Sheet & Protocol.pdf
