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5-Azacytidine 5-氮杂胞苷
Synonym
5-氮杂胞苷;5-Azacytidine;Ladakamycin;5-AZCR;Aza-C;
4-Amino-1-(β-D-ribofuranosyl)-1,3,5-triazin-2(1H)-one
Product Description
Molecular Formula: C8H12N4O5
Molecular Weight: 244.2
CAS Number: 320-67-2
Melting Point: approximately
Product Number: A 2385
Storage Temperature:
This product is a nucleoside analog, which can selectively activate eukaryotic gene expression and in some cases alter the differentiated state of some eukaryotic cells.1 It is a pyrimidine ring analog of cytidine. Its incorporation into RNA alters RNA synthesis and processing, and results in inhibition of protein synthesis.2
It is also thought to act by inhibiting enzymes that methylate cytosine residues in eukaryotic DNA.3,4 It activates genes by changing their methylation status, but may also act as a cytotoxin. It has been used as a cancer chemotherapeutic agent.1 This product is a powerful bacteriostatic, antitumor, and mutagenic agent;5 it also exhibits immunosuppressive, antimitotic, radioprotective, and virostatic effects.6 The cytostatic effect of this product has been directed against leukemia7 and it has been used in the treatment of solid tumors.8 It is reported that this product induces marked changes in the differentiated state of cultured mouse embryo cells9 and also inhibits the methylation of newly synthesized DNA.1
This product has also been used in cell cycle-specific reactivation of an inactive X-Chromosome locus10 and to study changes in phenotypic expression in embryonic and adult cells11 as well as in 10T1/2 and 3T3 cell lines.12
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions
This product is soluble in acetic acid:water (1:1 v/v) (5 mg/ml). It is also soluble in tissue culture medium (1.2 mg/10 ml). Once dissolved, this stock solution can then be diluted further in aqueous medium.
Storage/Stability
Azacitidine is very unstable in aqueous solutions, with a 10% loss of the product in 2 to 3 hours at room temperature in lactated Ringer’s solution.13 Degradation is complex, with an initial reversible formation of an intermediate formyl product, which then undergoes slower irreversible change to produce further degradation products. Loss of azacytidine is
rapid initially, but as the intermediate accumulates, reversal of the first reaction slows down the apparent loss of azacytidine. Misunderstanding of the kinetics has led to erroneously long (90%) stability times being quoted in the literature.14,15 The half-life of the compound in phosphate buffered saline, pH 7.4, when heated to
when kept at this temperature for 20 hours, lost the ability to cause cytotoxicity and multinucleation. Stock solutions should be prepared fresh for each experiment, sterilized by filtration, and kept at
Ordering Information
Description | Quantity | Price(RMB) | 促销价（RMB） | Cat. no. | 产地 |
5-Azacytidine | 100mg | 511 | 358 | A2385 | Sigma |
100mg*2 | 986 | 690 | A2385 | Sigma | |
100mg*10 | 3191 | 2393 | A2385 | Sigma |
