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Anti-Phosphor-PPP3CA (Ser469)抗体
| 产品编号 | YB-10343R |
| 英文名称 | Phosphor-PPP3CA (Ser469) |
| 中文名称 | ***酸化钙调***酸酶A/钙依赖***酸酶A抗体 |
| 别 名 | PPP3CA(Phosphor S469); PPP3CA(Phosphor Ser469); Calcineurin A alpha; CalcineurinA; Calcineurin A; Calmodulin dependent calcineurin A subunit alpha isoform; CALN; CALNA 1; CALNA; CALNA1; CAM PRP catalytic subunit; CCN 1; CCN1; CNA 1; CNA; CNA1; PPP2B; PPP3CA; Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform; Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform; PP2BA_HUMAN; CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform. |
| 规格价格 | 100ul/1580元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | ***酸化抗体 |
| 研究领域 | 细胞生物 免疫学 神经生物学 信号转导 激酶和***酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Cow, Rabbit, Guinea Pig, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 57kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PPP3CA around the phosphorylation site of Ser469:421-521/521 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively). Function: Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. Subcellular Location: Nucleus. Note=Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. Tissue Specificity: Interacts with CHP1 and CHP2. Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity. Interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. Interacts with DNM1L; the interaction dephosphorylates DNM1L and regulates its translocation to mitochondria. Interacts with CMYA5; this interaction represses calcineurin activity in muscle. Similarity: Belongs to the PPP phosphatase family. PP-2B subfamily. SWISS: Q08209 Gene ID: 5530 Database links: Entrez Gene: 5530 Human Entrez Gene: 19055 Mouse Entrez Gene: 24674 Rat Omim: 114105 Human SwissProt: Q08209 Human SwissProt: P63328 Mouse SwissProt: P63329 Rat Unigene: 435512 Human Unigene: 331389 Mouse Unigene: 6866 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP3CA (Ser469)) Polyclonal Antibody, Unconjugated (bs-10343R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
