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2X SG Fast qPCR 预混液
附带条件
特技
2XSG快速qPCR主混合物是一种即刻使用的鸡尾酒含有抗体介导的快速热启动Taq DNA聚合酶，SYBR Green I荧光染料，MgCl 2，DNTP混合物和一些稳定剂。它提供了一个2X主混合物，包含所有的成分，除了引物和模板的扩增和检测DNA的实时qPCR。抗体介导的快速热启动Taq DNA聚合酶和专有缓冲系统减少了引物二聚体的形成，增强了扩增特异性和效率，保证了Syr Green I实时QPCR的准确性。此外，SG快速QPCR主混合体中的SyBR GreenⅠ对实时PCR具有特异性，对热启动Taq DNA聚合酶的酶活性有一定的抑制作用。因此，Syr GreenⅠ的最佳浓度保证了实时荧光定量PCR中荧光强度的增强和检测灵敏度的提高。总之，SG快速QPCR主混剂具有良好的扩增效率和检测灵敏度。DNF缓冲液对存在多个非特异性扩增的引物是特异性的。对于一些DNA序列没有良好的引物，DNF缓冲液可以按照以下方案加入到反应混合物中。ROX参考染料不包括在SG快QPCR主混合中，适用于实时PCR仪器，不需要ROX参考标准化，如转子基因？DNA引擎OpTICON？Opticon？2，铬4？实时探测器，Mastercycler？EP RealPLEX，智能循环器？Roche LightCycler？480，Roche LightCycler？纳米，生物RAD CFX96，和Illumina ECO？.
特技
2×SG快速QPCR主混合物是一种现成的鸡尾酒，它只添加引物和模板来建立反应混合物。
热启动Taq DNA聚合酶和最佳缓冲体系保证了扩增的特异性和高效性。
在2×SG快速qPCR主混合物中，热启动Taq DNA聚合酶具有比普通热启动Taq DNA聚合酶更快的延伸率，使得实时qPCR的总反应时间更短。
独特的DNF缓冲液对存在多个非特异性扩增的引物具有特异性。
共有三种2×SG快速qPCR主混合物，包括NO /低/高ROX参考，适用于所有的实时PCR仪器。
船首
一个词
特赦
2XSG快速qPCR主混合物；DNF缓冲液；灭菌DDH2O。
概述
The 2xSG Fast qPCR Master Mix is a ready-to-use cocktail containing antibody-mediated fast hot start Taq DNA Polymerase, SYBR Green I fluorescent dye, MgCl2, dNTP Mix and some stabilizers. It is supplied as a 2X Master Mix containing all components except primers and template for the amplification and detection of DNA in real time qPCR. The antibody-mediated fast hot start Taq DNA Polymerase and proprietary buffer system reduce the formation of the primer dimers and enhance the amplification specificity and efficiency, which ensure the accuracy of the SYBR Green I real time qPCR. In addtion, the SYBR Green I in the SG Fast qPCR Master Mix is special for the real time PCR and has a little inhibition to the enzyme activity of the hot start Taq DNA Polymerase. So the optimal concentration of the SYBR Green I ensure the strong fluorescent intensity and improve the detection sensitivity in the real time qPCR. In brief, the SG Fast qPCR Master Mix has good amplification efficiency and detection sensitivity. The DNF Buffer is specific for the primers which exist multi nonspecific amplification. For some DNA sequences there are no good primers, the DNF Buffer can be added to the reaction mix according to the following protocol. The ROX reference dye is not included in the SG Fast qPCR Master Mix and applicable to the real time PCR instruments that are no need to normalization by the Rox reference, like Rotor-Gene?, DNA Engine Opticon?, Opticon? 2, Chromo 4? Real-Time Detector, Mastercycler? ep realplex, Smart Cycler?, Roche LightCycler? 480, Roche LightCycler? Nano, Bio-Rad CFX96, and Illumina Eco?.
特点
- The 2×SG Fast qPCR Master Mix is a ready-to-use cocktail, which only add primers and template to set up the reaction mix.
- The hot start Taq DNA Polymerase and optimal buffer system ensure the high amplification specificity and efficiency.
- The hot start Taq DNA Polymerase in the 2×SG Fast qPCR Master Mix has faster extension rates than common hot start Taq DNA Polymerase，making the total reaction time of the real time qPCR shorter.
- The unique DNF Buffer is specific for the primers which exist multi nonspecific amplification.
- There are three kinds of 2×SG Fast qPCR Master Mix including no/low/high Rox reference，applying to all the the real time PCR instruments.
应用
基因表达分析、拷贝数分析
组份
2xSG Fast qPCR Master Mix; DNF Buffer; Sterilized ddH2O.
