DeoxyUridine (dU) can be substituted for dT in DNA oligonucleotides. The base can be removed by the enzyme uracil-Ndeglycosylase (UNG), which renders the oligo susceptible to strand scission. One common use of this strategy is to elimina...
DeoxyUridine (dU) can be substituted for dT in DNA oligonucleotides. The base can be removed by the enzyme uracil-Ndeglycosylase (UNG), which renders the oligo susceptible to strand scission. One common use of this strategy is to elimina...
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