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Polyclonal antibody to anti mouse Nanog
Back Ground
Transcriptional factors, OCT3/4 (POU5F1) and STAT3 function as key regulators in maintaining
pluripotency of stem cells. Thus, POU5F1 and STAT3 have been widely used as molecular markers
of pluripotential stem cells. Pluripotential cell-specific Nanog gene is a newly identified
homeodomain-bearing transcriptional factor
1),2)
. Importantly, Nanog is expressed specific to early
embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and
embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining
the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway.
Therefore, Nanog is one of the molecular markers suitable for recognizing the undifferentiated state
of stem cells in the mouse and human.
Reference
1. Mitsui,K.et al. : Cell.2003 May 30;113(5):631-642
2. Hatano, S.Y., Tada, M., Kimura, H., Yamaguchi, S., Kono, T., Nakano, T.,
Suemori, H., Nakatsuji, N. and Tada, T. (2005)
Pluripotential competence of cells associated with Nanog activity.
Mech Dev 122, 67-79.
Details
Form: affinity purified polyclonal antibody, Liquid
Immunogen: Mouse Nanog protein
Cross Reactivity: Mouse,Human Monkey
Concentration: 0.2mg/ml
Storage Buffer: PBS,pH7.4(0.1% sodium azide as preservatives)
Storage condition: -4C°~-20C°
Applications: Mouse / Immunocytochemistry, Immunoprecipitation
Western blot
Human,Monkey / Immunocytochemistry
Application Notes:
Western blot: Use at a dilution of 1/300 – 1/2000
(with PBS containing 0.1% Triton X-100,1% BSA,
or TBS Containing 3% skim milk.)
Immunocytochemistry: Use at a dilution of 1/150 – 1/700
(with PBS containing 0.1% Triton X-100,1% BSA,
10% goat serum, Mouse embryonic atem cells were
fixed with 3.7% formaldehyde in PBS)
Immunoprecipitation: Use at a dilution of 1/300
(with RIPA buffetr)
