MCAO栓线简介
MCAO栓线（MCAO Monofilaments）是制备大鼠/小鼠（或其它实验动物）局部脑缺血模型的非常关键的实验材料，本产品是用柔韧适度的单丝尼龙线，经显微操作烧制而成，其头端光滑、大小一致，易进入颅内又不至于刺破血管，使用本产品可大大提高模型制备的成功率，及脑缺血范围的稳定性。本产品已被美国、加拿大、香港、中国大陆等的科研机构、大学、医院广泛采用。研究论文发表于本专业顶级的期刊：J Neuroscience，Stroke，Journal of Cerebral Blood，Flow & Metabolism，Journal of Neurochemistry等（见附录）。
产品分五个级别，每个级别分若干型号和规格。（见MCAO栓线型号/价格表）
A1级：头端烧熔为半球形（图1），无包被、无标记、未消毒，需要用户自己消毒、做标记；
A2级：头端烧熔为半球形，无包被、有标记*、已消毒，可以直接使用；
A3级：头端烧熔为半球形，前端包被多聚赖氨酸#、无标记、已消毒，需要用户自己做标记；
A4级：头端烧熔为半球形，前端包被多聚赖氨酸、有标记、已消毒，即买即用型，不需任何处理。
A5级：头端烧熔为半球形，且前端5-6mm均匀包被硅橡胶（图2），有标记、已消毒，即买即用型，不需任何处理。包被硅胶的直径及对应的动物体重见附表。如客户订做小鼠的新型号硅胶线（指硅胶直径>0.22的型号），则每次最少10根/400元，如超过30根，仍按35元/根；如客户订做大鼠的新型号硅胶线（指硅胶直径>0.28的其它型号），则每次最少10根/300元，如超过30根，仍按25元/根。A5栓线插线操作相对难于其它级别，建议预实验时，另选一个相邻的较小体重级别的型号，如大鼠体重为240-270g，除了选2634型号的硅胶线，最好再选一个对应200-240g体重的型号。另外还可以选硅胶长度为3-4mm的硅胶线，相对好操作一些。
* 大鼠线栓的标记在距头端19-20mm处，小鼠线栓的标记在距头端9-10mm处.
# 前端20mm(大鼠线栓)或10mm(小鼠线栓)包被多聚-L-赖氨酸（包被多聚-L-赖氨酸不会改变栓线的直经）。栓线包被多聚-L-赖氨酸，可以提高MCAO模型的稳定性。
最新MCAO栓线型号/价格表，请致电13301307459或4008636007，或发邮件ysf_ok@sina.com, mcaomodel@163.com
附录：使用我们的MCAO栓线最近发表的部分SCI英文论文
（论文中的“Beijing Sunbio Biotech Co. Ltd.”既是西浓科技的前身）
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Acetylbritannilactone Modulates MicroRNA-155-Mediated Inflammatory Response in Ischemic Cerebral Tissues. Mol Med. 2015; 21(1): 197–209.6. Yao H, Gao M, Ma J, et al. Transdifferentiation-Induced Neural Stem Cells Promote Recovery of Middle Cerebral Artery Stroke Rats. PLoS One. 2015; 10(9): e0137211.7. Fang L, Gao H, Zhang W, et al. Resveratrol alleviates nerve injury after cerebral ischemia and reperfusion in mice by inhibiting inflammation and apoptosis. Int J Clin Exp Med. 2015; 8(3): 3219–3226.8. Hua K, Sheng X, Li TT, et al. The edaravone and 3-n-butylphthalide ring-opening derivative 10b effectively attenuates cerebral ischemia injury in rats. Acta Pharmacol Sin. 2015 Aug; 36(8): 917–927.9. Lan Z, Xu X, Xu W, et al. Discovery of 3-n-butyl-2,3-dihydro-1H-isoindol-1-one as a potential anti-ischemic stroke agent. Drug Des Devel Ther. 2015; 9: 3377–3391.10. Li X, Li C, Wei LY, et al. 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Journal of Cerebral Blood Flow & Metabolism (2013) 33, 253–262; 25. An S, Kuang YY, Shen T, et al. Brain-targeting delivery for RNAi neuroprotection against cerebral ischemia reperfusion injury. Biomaterials 34 (2013) 8949-8959.26. Fu J, Xue R, Gu JF, et al. Neuroprotective effect of calcitriol on ischemic/reperfusion injury through the NR3A/CREB pathways in the rat hippocampus. Molecular Medicine Reports. 2013, 1708-1714.27. Cheng FF, Lu Y, Zhong XG. Baicalins Therapeutic Time Window of Neuroprotection during Transient Focal Cerebral Ischemia and Its Antioxidative Effects In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine, vol.2013, PP.1-11, 2013.28. Chen XY, Sun XC, Su HX. Upregulation of myeloid cell leukemia-1 potentially modulates beclin-1-dependent autophagy in ischemic stroke in rats. BMC Neuroscience, 2013, 14:56.29. Dong BB, Cai M, Fang ZP. Hemopexin induces neuroprotection in the rat subjected to focal cerebral ischemia. BMC Neuroscience 2013, 14:58.30. Lan R, Xiang J, Zhang Y. PI3K/Akt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats. Evidence-Based Complementary and Alternative Medicine, Volume 2013.31. Li JJ, Sheng WL, Feng CZ. Pyrrolidine dithiocarbamate attenuates brain Aβ increase and improves long-term neurological outcome in rats after transient focal brain ischemia. Neurobiol Dis. 2012; 45(1): 564–572.32. Guo JY, Ding J, Yuan F. Dose-Dependent Protective Effect of Bisperoxovanadium against Acute Cerebral Ischemia in a Rat Model of Ischemia/Reperfusion Injury. Int. J. Mol. Sci. 2013, 14, 12013-12022. 33. Li LL, Zuo ZY. Isoflurane postconditioning induces neuroprotection via Akt activation and attenuation of increased mitochondrial membrane permeability. Neuroscience. 2011 December 29; 199: 44–50. 34. Li H, Yin JB, Li LL, et al. 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