Anti-14-3-3 theta抗体_流式抗体_抗体_免疫抗体库_商城

特异性ab183075 detects endogenous level of total 14-3-3 theta protein.
经测试应用WB, IHC-Pmore details
种属反应性
与反应: Mouse, Human 预测可用于: Rat, Cow, Orangutan
免疫原
Synthetic peptide within Human 14-3-3 theta aa 140-154. The exact sequence is proprietary. Genbank: NP_006817 Sequence:

QTIDNSQGAYQEAFD
Database link: P27348

Run BLAST with Run BLAST with
阳性对照
- Mouse brain, Human lymphoma and Human colon cancer tissue lysates; Human A549 and mouse NIH 3T3 cell lysates; Human tonsil tissue

形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存��溶液pH: 7.30 Preservative: 0.05% Sodium azide Constituents: 50% Glycerol, 49% PBS PBS is without Mg²⁺ and Ca²⁺
浓度100 µl 浓度为 0.6 mg/ml
纯度Immunogen affinity purified
克隆多克隆
同种型IgG
研究领域
- Signal Transduction
- Adapters
- Cytoplasmic
- Stem Cells
- Signaling Pathways
- Wnt
- 14-3-3

Our Abpromise guarantee covers the use of ab183075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Abreviews	说明
WB		1/300 - 1/2000. Predicted molecular weight: 28 kDa.
IHC-P		1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

细胞定位Cytoplasmic
数据库链接
- Entrez Gene: 768311 Cow
- Entrez Gene: 10971 Human
- Entrez Gene: 22630 Mouse
- Entrez Gene: 100171925 Orangutan
- Entrez Gene: 25577 Rat
- Omim: 609009 Human
- SwissProt: Q3SZI4 Cow
- SwissProt: P27348 Human
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别名
- 14-3-3 antibody
- 14-3-3 protein T-cell antibody
- 14-3-3 protein tau antibody
see all

Western blot - Anti-14-3-3 theta antibody (ab183075)

All lanes : Anti-14-3-3 theta antibody (ab183075) at 1/300 dilution Lane 1 : mouse brain tissue lysate Lane 2 : Human A549 cell lysate Lane 3 : Human lymphoma tissue lysate Lane 4 : mouse NIH 3T3 cell lysate Lane 5 : Human colon cancer tissue lysate Lysates/proteins at 40 µg per lane. Secondary Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution developed using the ECL technique Predicted band size : 28 kDa Exposure time : 5 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 theta antibody (ab183075)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 14-3-3 theta with ab183075 at a 1/20 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)

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