Anti-14-3-3 delta + zeta (phospho T232)抗体_流式抗体_抗体_免疫抗体库_商城

特异性ab195909 detects endogenous levels of 14-3-3 delta + zeta only when phosphorylated at Threonine 232.
经测试应用WBmore details
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide corresponding to Human 14-3-3 delta + zeta (phospho T232). (Derived from around the phosphorylation site of Threonine 232). Database link: P63104
阳性对照
- UV treated Jurkat whole cell lysate.

形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol, 49% PBS, 0.87% Sodium chloride
浓度100 µg 浓度为 1 mg/ml
纯度Immunogen affinity purified
克隆多克隆
同种型IgG

Our Abpromise guarantee covers the use of ab195909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Abreviews	说明
WB		1/500 - 1/2000. Predicted molecular weight: 28 kDa.

功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
序列相似性Belongs to the 14-3-3 family.
翻译后修饰The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53.
细胞定位Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
Information by UniProt
数据库链接
- Entrez Gene: 7534 Human
- SwissProt: P63104 Human
- Unigene: 492407 Human
别名
- 14-3-3 protein zeta/delta antibody
- 1433Z_HUMAN antibody
- KCIP-1 antibody
see all

Western blot - Anti-14-3-3 delta + zeta (phospho T232) antibody (ab195909)

All lanes : Anti-14-3-3 delta + zeta (phospho T232) antibody (ab195909) at 1/500 dilution Lane 1 : UV treated Jurkat whole cell lysate with antigen specific peptide Lane 2 : UV treated Jurkat whole cell lysate Lysates/proteins at 12.5 µg per lane. Predicted band size : 28 kDa

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