山羊Anti-Rabbit IgG Fc (HRP)_流式抗体_抗体_免疫抗体库_商城

靶标种属Rabbit
特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG.
经测试应用ICC, IHC-P, ELISA, WBmore details
偶联物HRP

形式Liquid
存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.1% Proclin Constituents: 0.2% BSA, PBS
浓度1 mg 浓度为 1 mg/ml
纯度Immunogen affinity purified
纯化说明Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
共轭说明Molar enzyme/ antibody protein ratio is 4:1
克隆多克隆
同种型IgG
研究领域
- Immunology
- Immunoglobulins
- Heavy Chain
- IgG
- Secondary antibodies
- anti-Rabbit
- IgG
- Enzyme
- HRP

Our Abpromise guarantee covers the use of ab97200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Abreviews	说明
ICC		Use at an assay dependent dilution.
IHC-P		1/200 - 1/2000.
ELISA		1/10000 - 1/100000. (Primary ELISA)
WB		1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG Fc (HRP) (ab97200)

IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97200, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Goat anti-Rabbit IgG Fc (HRP) secondary antibody (ab97200)

Ab97200 was used at dilution 1/5000 with the primary antibody ab90045 in WB. See the review on ab90045.

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