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Anti-14-3-3 epsilon抗体
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单价： ￥2100.00
品牌： abcam
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更新： 2021-07-01
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  • 特异性This antibody detects endogenous level of total 14-3-3 epsilon protein.
  • 经测试应用ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, African Green Monkey
  • 免疫原

    Recombinant full length protein corresponding to Human 14-3-3 epsilon aa 1-255. Sequence:

    MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYK NVIGARRASW RIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICC DILDVLDKHLIPAANTGESKVF YYKMKGDYHRYLAEFATGNDRKEAAE NSLVAYKAASDIAMTELPPTHPIRLGLALNFSVF YYEILNSPDRACRL AKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGE EQ NKEALQDVEDENQ

    Database link: P62258

     

    Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • T47D, Jurkat, PC12, COS1, COS7, NIH3T3 and SW480 cell extracts.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride without Mg2+ and Ca2+
  • 浓度100 µl 浓度为 1 mg/ml 
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域
    • Neuroscience
      •  
    • Neurology process
      •  
    • Neural Signal Transduction
    • Signal Transduction
      •  
    • Adapters
      •  
    • Cytoplasmic
    • Neuroscience
      •  
    • Cell Type Marker
      •  
    • Neuron marker
      •  
    • Synapse marker
    • Stem Cells
      •  
    • Signaling Pathways
      •  
    • Wnt
      •  
    • 14-3-3

应用

Our Abpromise guarantee covers the use of ab196891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Abreviews 说明
ICC/IF   1/20 - 1/100.
IHC-P   1/50 - 1/100.
WB   1/500 - 1/2000. Predicted molecular weight: 29 kDa.

靶标

  • 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
  • 序列相似性Belongs to the 14-3-3 family.
  • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
    • Entrez Gene: 7531 Human
    • Entrez Gene: 22627 Mouse
    • Entrez Gene: 29753 Rat
    • Omim: 605066 Human
    • SwissProt: P62258 Human
    • SwissProt: P62259 Mouse
    • SwissProt: P62260 Rat
    • Unigene: 513851 Human
    see all
  • 别名
    • 14 3 3 E antibody
    • 14 3 3 epsilon antibody
    • 14 3 3E antibody
    see all

Anti-14-3-3 epsilon antibody 图像

  • Western blot - Anti-14-3-3 epsilon antibody (ab196891)
    Western blot - Anti-14-3-3 epsilon antibody (ab196891)
    All lanes : Anti-14-3-3 epsilon antibody (ab196891) at 1/500 dilution Lane 1 : T47D cell extract Lane 2 : Jurkat cell extract Lane 3 : PC12 cell extract Lane 4 : COS1 cell extract Lane 5 : COS7 cell extract Lane 6 : NIH 3T3 cell extract Lane 7 : SW480 cell extract Predicted band size : 29 kDa