点击图片查看原图
- 经测试应用WB, ELISAmore details
- 免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Polyethylene glycol conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponds to Polyethylene glycol 20K (PEG20K).
- 表位This antibody recognizes the backbone of the Polyethylene glycol molecule.
- 常规说明
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
ab133471 is useful in measuring the pharmacokinetics of Polyethylene glycol modified molecules. This antibody is specific to the backbone of PEG molecule. For detection of PEG methoxy group, see Anti-Polyethylene glycol antibody [PEG-B-47] - ab51257
For PEG specific ELISA kit - see Polyethylene Glycol (PEG) RabMAb ELISA Kit - ab138914
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents when performing ELISA using anti-PEG products.
Antibody Isotype: Please note, this RabMAb products isotype is Rabbit IgM. Anti-IgM secondary is recommended as a secondary antibody. List of anti-Rabbit IgM secondaries.
Learn more about PEG RabMAb primary antibodies and kit - PEG RabMAb Product Portal Page
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
- 存储溶液pH: 7.20 Preservative: 0.01% Sodium azide Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
- 纯度Protein A purified
- Primary antibody说明ab133471 is useful in measuring the pharmacokinetics of Polyethylene glycol modified molecules.
- 克隆单克隆
- 克隆编号PEG-2-128
- 同种型IgM
- 研究领域
- Cell Biology
- Other Antibodies
- Drugs and Chemicals
应用
Our Abpromise guarantee covers the use of ab133471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Abreviews | 说明 |
|---|---|---|
| WB | 1/1000. The MW of the WB band for ab133471 varies based on the PEGylated molecule detected. | |
| ELISA | Use a concentration of 2 - 10 µg/ml. |
靶标
- 别名
- PEG antibody
Anti-Polyethylene glycol antibody [PEG-2-128] 图像
-
![Western blot - Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471)](https://www.infobio.cn/skin/demo08/image/lazy.gif)
Western blot - Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471)All lanes : Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471) at 1/1000 dilution (purified) Lane 1 : PEG20K Lane 2 : MBP-PEG20K Lysates/proteins at 5 µg per lane. Secondary Peroxidase-conjugated goat anti-rabbit IgM, (H+L) at 1/500 dilutionBlocking buffer: 15% BSA/TBS.
Dilution buffer: 10% PAA /TBST.
- ELISA - Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471)
Direct ELISA antigen dose-response curve using purified ab133471. Antigen concentration of 0-100 ng/mL. A HRP-conjugated goat anti-rabbit IgM (1/1000) was used as the secondary antibody.
- ELISA - Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471)
Direct ELISA antigen dose-response curve using purified ab133471. Antigen concentration of 0-10000 ng/mL. A HRP-conjugated goat anti-rabbit IgM (1/1000) was used as the secondary antibody.
- Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471)
Sandwich ELISA antigen dose-response curve using purified ab133471.
Capture antibody - ab133471 (10000 ng/ml).
Antigens - MBP-PEG20K and ADI-PEG (0-1000 ng/ml).
Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).
- Western blot - Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471)All lanes : Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471) at 1/1000 dilution (unpurified) Lane 1 : MBP-PEG20K at 1 µg Lane 2 : MBP-PEG20K at 0.5 µg Lane 3 : PEG20K at 1 µg Lane 4 : PEG20K at 0.5 µg Secondary HRP-conjugated goat anti-rabbit IgM at 1/2000 dilutionGel concentration: 4-20%
- ELISA - Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471)
Various concentrations of Polyethylene glycol polymers with amine end groups were coated in 96 wells. 2 ug/ml of unpurified ab133471 was added. After washing, samples were incubated with 1 ug/ml of HRP-conjugated anti-rabbit IgM.
- ELISA - Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471)
Various concentrations of Polyethylene glycol 20K (PEG20K) conjugated MBP and branched Polyethylene glycol conjugated BSA were coated in 96 wells. 2 ug/ml of unpurified ab133471 was added. After washing, samples were incubated with 1 ug/ml of HRP-conjugated anti-rabbit IgM.
- ELISA - Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471)
10 ug/ml of unpurified ab133471 was coated into 96 wells. Serial dilution of Polyethylene glycol 20K (PEG20K) conjugated MBP and PEGylated ADI were added. After washing, 1 ug/ml of biotinylated anti-Polyethylene glycol antibody was added. Streptavidin-HRP was then used to develop the color.
