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- 经测试应用WB, IHC-Pmore details
- 种属反应性 与反应: Mouse, Rat, Human 预测可用于: Sheep, Chicken, Cow
- 免疫原
Recombinant full length protein corresponding to Human 14-3-3 epsilon aa 2-254. (With tag; BC000179). Sequence:
DDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKN VIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDIL DVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVA YKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDD AIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVE DENQ
Database link: P62258Run BLAST withRun BLAST with - 阳性对照
- Mouse brain tissue lysate; HT29, 293T and HeLa cell lysates; Human colon cancer tissue.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
- 存储溶液pH: 7.30 Preservative: 0.05% Sodium azide Constituents: 49% PBS, 50% Glycerol Note: PBS without Mg2+ and Ca2+.
- 浓度100 µl 浓度为 0.8 mg/ml
- 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
- Neuroscience
- Neurology process
- Neural Signal Transduction
- Signal Transduction
- Adapters
- Cytoplasmic
- Neuroscience
- Cell Type Marker
- Neuron marker
- Synapse marker
- Stem Cells
- Signaling Pathways
- Wnt
- 14-3-3
应用
Our Abpromise guarantee covers the use of ab182656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Abreviews | 说明 |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 29 kDa. | |
| IHC-P | 1/25 - 1/150. |
靶标
- 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
- 序列相似性Belongs to the 14-3-3 family.
- 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 数据库链接
- Entrez Gene: 417554 Chicken
- Entrez Gene: 282125 Cow
- Entrez Gene: 7531 Human
- Entrez Gene: 22627 Mouse
- Entrez Gene: 29753 Rat
- Entrez Gene: 443300 Sheep
- Omim: 605066 Human
- SwissProt: Q5ZMT0 Chicken
see all - 别名
- 14 3 3 E antibody
- 14 3 3 epsilon antibody
- 14 3 3E antibody
- 14-3-3 protein epsilon antibody
- 14-3-3E antibody
- 1433E_HUMAN antibody
- Epididymis luminal protein 2 antibody
- FLJ45465 antibody
- FLJ53559 antibody
- HEL2 antibody
- KCIP 1 antibody
- KCIP1 antibody
- MDCR antibody
- MDS antibody
- Mitochondrial import stimulation factor L subunit antibody
- Protein kinase C inhibitor protein1 antibody
- Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon antibody
- Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide antibody
- Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide antibody
- YWHAE antibody
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Anti-14-3-3 epsilon antibody 图像
- Immunohistochemistry (Paraffin-embedded sections) - Anti-14-3-3 epsilon antibody (ab182656)
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue lableing 14-3-3 epsilon with ab182656 at 1/10 dilution (left panel). Right panel is treated with the immunogen.
- Western blot - Anti-14-3-3 epsilon antibody (ab182656)All lanes : Anti-14-3-3 epsilon antibody (ab182656) at 1/250 dilution Lane 1 : HT29 cell lysate Lane 2 : Mouse brain tissue lysate Lane 3 : 293T cell lysate Lane 4 : HeLa cell lysate Lysates/proteins at 30 µg per lane. Secondary Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution developed using the ECL technique Predicted band size : 29 kDa Exposure time : 1 second
10% SDS PAGE
