pCS2+8CmCherry载体_体外转录/翻译_克隆与表达_生化试剂库_商城

货号：	ZY
供应商：	泽叶生物
数量：	20
英文名：	pCS2+8CmCherry
保质期：	三年
保存条件：	-20℃低温保存

载体基本信息

出品公司:	ZYbscience
载体名称:	pCS2+8CmCherry
质粒类型:	克隆载体；哺乳动物表达载体；广宿主载体；体外表达载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	simian CMV IE94、lac、T3、SP6
载体大小:	4848 bp
5' 测序引物及序列:	SP6 fwd（MCS1）、T3 fwd（MCS2）
3' 测序引物及序列:	EBV rev（MCS1）、SV40 polyA rev（MCS2）
载体标签:	mCherry
载体抗性:	氨苄青霉素
克隆菌株:	DH10B
表达细胞:	原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。
备注:	pCS2+8CmCherry载体能够在多种细胞中进行高水平的瞬表达。 pCS2+8CmCherry载体同时也能进行体外转录和翻译。
产品目录号:	ZY34935
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

载体质粒图谱和多克隆位点信息

载体简介

载体序列

LOCUS pCS2+8CmCherry Sequencing Result	4848 bp 	DNA	SYNDEFINITION pCS2+8CmCherry Sequencing ResultCOMMENT This file is created by Vector NTI http://www.biofeng.com/COMMENT ORIGDB|GenBankCOMMENT VNTAUTHORNAME|biofeng.com|COMMENT VNTNAME|pCS2+8CmCherry|FEATURES Location/Qualifiers source 1..4848 /organism="pCS2+8CmCherry Sequencing Result" /mol_type="other DNA" misc_feature 186..208 /label="M13_pUC_rev_primer" promoter 207..225 /label="M13_reverse_primer" promoter 242..261 /label="T3_promoter" misc_feature 464..483 /label="EBV_rev_primer" CDS complement(558..1268) /label="ORF frame 2" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ YERAEGRHSTGGMDELYK*" gene complement(561..1265) /label="mCherry" /gene="mCherry" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ YERAEGRHSTGGMDELYK*" misc_feature complement(1035..1088) /label="Q66M (mCherry)" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ YERAEGRHSTGGMDELYK*" misc_feature complement(1355..1372) /label="Sp6_primer" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ YERAEGRHSTGGMDELYK*" misc_feature complement(1448..1489) /label="CMV_fwd_primer" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ YERAEGRHSTGGMDELYK*" CDS complement(1958..2482) /label="ORF frame 3" /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA LCHIVFHIWVFLLT*" rep_origin 2479..2785 /label="f1_origin" /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA LCHIVFHIWVFLLT*" promoter 2978..3006 /label="AmpR_promoter" /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA LCHIVFHIWVFLLT*" gene 3048..3908 /label="Ampicillin" /gene="Ampicillin" /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA LCHIVFHIWVFLLT*" CDS 3048..3908 /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 4063..4682 /label="pBR322_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgCAGCTGGC 61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTCCC GGCTCGTATG TTGTGTGGAA 181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG 241 CAATTAACCC TCACTAAAGG GAACAAAAGC TGGGTACCGG GCCCAATGCA TTGGCGCCGC 301 GGCCGCGAAT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA 361 ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC 421 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC 481 ATCAATGTAT CTTATCATGT CTGGATCTAC GTAATACGAC TCACTATAGT TCTAGAGGCT 541 CGAGAGGCCT TGAATTCTTA CTTGTACAGC TCGTCCATGC CGCCGGTGGA GTGGCGGCCC 601 TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAAC 661 TTGATGTTGA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT GTAGGTGGTC 721 TTGACCTCAG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT GATCTCGCCC 781 TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC CATGGTCTTC 841 TTCTGCATTA CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC CTTGTAGATG 901 AACTCGCCGT CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC GTCCTCGAAG 961 TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA GTCGGGGATG 1021 TCGGCGGGGT GCTTCACGTA GGCCTTGGAG CCGTACATGA ACTGAGGGGA CAGGATGTCC 1081 CAGGCGAAGG GCAGGGGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG 1141 TAGGGGCGGC CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC GGAGCCCTCC 1201 ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGATGGCCA TGTTATCCTC CTCGCCCTTG 1261 CTCACCATCA ATCGATGTCC GGCGATCGCT GGCCGGCCTT TAATTAATGG CGCGCCACTA 1321 GTGGATCCTG CAAAAAGAAC AAGTAGCTTG TATTCTATAG TGTCACCTAA ATCAAGCTTG 1381 CTCCGACGTC CCCAGGCAGA ATGGCGGTTC CCTAAACGAG CATTGCTTAT ATAGACCTCC 1441 CATTAGGCAC GCCTACCGCC CATTTACGTC AATGGAACGC CCATTTGCGT CATTGCCCCT 1501 CCCCATTGAC GTCAATGGGG ATGTACTTGG CAGCCATCGC GGGCCATTTA CCGCCATTGA 1561 CGTCAATGGG AGTACTGCCA ATGTACCCTG GCGTACTTCC AATAGTAATG TACTTGCCAA 1621 GTTACTATTA ATAGATATTG ATGTACTGCC AAGTGGGCCA TTTACCGTCA TTGACGTCAA 1681 TAGGGGGCGT GAGAACGGAT ATGAATGGGC AATGAGCCAT CCCATTGACG TCAATGGTGG 1741 GTGGTCCTAT TGACGTCAAT GGGCATTGAG CCAGGCGGGC CATTTACCGT AATTGACGTC 1801 AATGGGGGAG GCGCCATATA CGTCAATAGG ACCGCCCATA TGACGTCAAT AGGAAAGACC 1861 ATATATAGAG ACCATTGACG TCAATGGGGG AGTGGCTATG GGCGGTATTA GGAAGCCCCA 1921 TATATGGTAT ATGGGACCGC CCATTGGGAG GGGCTATCTA CGTCAATAGG AAAACCCATA 1981 TATGGAATAC TATATGGCAT AGGGCCAATA CATAGTATTG AACCTGGCCA ATAGCCATAT 2041 TGGCATAGGG CCATATTGGA TATTGCCTAT ATATTGATCC TGGCATATAG CCAATATGGC 2101 CGCCATTATT GGCACCATGC CAATTCAATA TGGCGGACTT GGCACAGGGC CAAGACCCCT 2161 CCTCAAACTT GGCACCGTGC CAAGTAGACC CCTCCCCAGT TGGCACAGTG CCAGGTCCGC 2221 CATATTGAAT TGGCATGGTG CCAAGTCCGC CATATTGAAT TGGCTGGGGT CCAGATCCAC 2281 CATATTGAAT TGGCACGAGT CCATATACGC CATATTGAAT TGGCACAGGT CCAGATCCAC 2341 CATATTGAAT TGGCATGAGT CCATATACGC CATATTGAAT TGGCGGTCGA CTGGCGTAAT 2401 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 2461 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 2521 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 2581 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 2641 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 2701 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 2761 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 2821 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 2881 AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG 2941 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA 3001 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC 3061 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC 3121 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA 3181 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC 3241 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG 3301 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC 3361 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA 3421 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG 3481 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC 3541 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG 3601 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT 3661 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG 3721 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG 3781 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC 3841 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC 3901 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT 3961 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT 4021 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT 4081 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG 4141 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA 4201 GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA 4261 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG 4321 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG 4381 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT 4441 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA 4501 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC 4561 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG 4621 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG 4681 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT 4741 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC 4801 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAG//

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