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份 库存999份
| 货号： | GT707 |
GV3101感受态细胞
GV3101 Competent Cells
Transformation Protocol
Freeze-thaw method
1Add about 1 ug of plasmid DNA to cells.
2 Freeze the cells in liguid nitrogen
3Thaw the cells by incubating the test tubes in a 37℃ water bath for 5 min.
4 Add 1ml of Yep medium to the tube and incubate at 28℃ for 2-4H with gentle shaking. This period allows the bacteria to express the antibiotic resistance genes.
5 Centrifuge the tubes for 30s in an Eppendorf centrifuge .Discard the supernatant solution. Resuspend the cells in 0.1ml YEP medium.
6 Spread the cells on a YEP agar plate containning appropriate antibiotic selection .
Incubate the plate at 28℃ 。Transformed colonies shoud appear in 1-3days.
GV3101 Competent Cells
| Catalog | Name | Desciption | 当日秒杀价 |
| Waryong GT707 | GV3101 competent cell,200ul*10 | Resistance:Rifampicin 20ug/ml | 2380.00 |
Freeze-thaw method
1Add about 1 ug of plasmid DNA to cells.
2 Freeze the cells in liguid nitrogen
3Thaw the cells by incubating the test tubes in a 37℃ water bath for 5 min.
4 Add 1ml of Yep medium to the tube and incubate at 28℃ for 2-4H with gentle shaking. This period allows the bacteria to express the antibiotic resistance genes.
5 Centrifuge the tubes for 30s in an Eppendorf centrifuge .Discard the supernatant solution. Resuspend the cells in 0.1ml YEP medium.
6 Spread the cells on a YEP agar plate containning appropriate antibiotic selection .
Incubate the plate at 28℃ 。Transformed colonies shoud appear in 1-3days.
| stbl3感受态细胞 LBA4404感受态细胞 XL1-Blue感受态细胞 DH10Bac感受态细胞 ER2566感受态细胞 BJ5183感受态细胞 DB3.1感受态细胞 JM110感受态细胞 GV3101感受态细胞 SURE感受态细胞 SOC培养基，SOB培养基，LB培养基，配制方法，AT克隆试剂盒 BL21(DE3)感受态细胞 TB1感受态细胞 EGY48酵母菌感受态细胞 EGY48感受态细胞 AH109酵母感受态细胞 | HMS174 Y187酵母感受态细胞 XL2-Blue感受态细胞 平板涂布专用玻璃珠 stbl3感受态细胞 NMY51酵母感受态细胞 TG1感受态细胞，TG1感受态细胞 EHA105感受态细胞 Stbl2感受态细胞 DH10B感受态细胞 T1感受态细胞 sure感受态细胞 AGL1农杆菌感受态细胞 M15感受态细胞 HB101感受态细胞 JM109感受态细胞 Y1HGold酵母感受态细胞 Rosseta(DE3)感受态细胞 | TG1感受态细胞 XL10-Gold 感受态细胞 农杆菌感受态细胞 大肠杆菌感受态细胞 DH5α感受态细胞 TOP10感受态细胞 C43, C43(DE3)感受态细胞 Mach1-T1 Phage Resistant 感受态细胞OrigamiB,OrigamiB(DE3),OrigamiB(DE3)pLysS,OrigamiB(DE3)pLacI C41(DE3), C41(DE3)PlysS感受态细胞 Y2HGold 感受态细胞 MC1061感受态细胞 EHA103感受态细胞 RN4220感受态细胞 ER2738感受态细胞 B834，B834(DE3) ，B834(DE3)pLysS AD494感受态细胞 BLR感受态细胞 DMT感受态细胞 TKB1感受态细胞 重组金黄色葡萄球菌蛋白A Origami感受态细胞 |
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