分子伴侣系统pTf16

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 载体系统

杂交系统:

哺乳动物双杂交系统

Promega2500

VectorspACTpBIND

Contorl plasmidpACT-MyoDpBIND-Id

Reporter plasmidpG5 luc

Stratagene3500

VectorspCMV-BDpCMV-AD

Contorl plasmidpBD-p53pBD-NF−κBpAD-SV40TpAD-TRAF

Reporter plasmidpFR-luc

酵母双杂交系统

Clontech2800

VectorspGBKT7pGADT7

Positive contorlpCL1pGBKT7-53pGADT7-T

Negative controlpGBKT7-Lam

StrainAH109

VectorpACT2 AD

Stratagene1500

VectorspSospMyr

Control plasmidpSos MAFB

Strain

酵母单杂交系统

Clontech800

VectorpHis2

酵母三杂交系统

Clontech1500

VectorpBridge

StrainsY2H Gold Yeast strainY187

诱导系统:

蜕皮***诱导表达系统

Invitrogen1200

VectorspINDpVgRxR

LacSwith II哺乳动物诱导表达系统

Stratagene1800

VectorspOPRSVIpOPI3CATpCMVLacI

GeneSwith 哺乳动物诱导系统

Invitrogen1200

VectorspSwitchpGene v5-His B (BamHI-EcoRI)

四环素诱导系统

Invitrogen2500

VectorspcDNA4/TO/Myc-His ApcDNA4/TO/Myc-His BpcDNA4/TO/Myc-His C

Contorl plasmidpcDNA4/TO/Myc-His/LacZ

Regulatory plasmidpcDNA6/TR

Clontech3600

VectorspTet-OnpTet-OffpTRE2pTRE2 hygromcs区含有luciferase片段pTK-hyg

         pRevTet-OnpRevTet-OffpRevTRE

         pTet on advancedpTRE TightpTRE Tight Luciferase(一套2400

        pCMV-Tet3GpTRE3GpTRE3G-Luc(一套3000

 

Tale ToolBox kit TALEN and TALETF):8000

VectorspNI v2pNG v2pNN v2pHD v2pTALETF v2 (NN)pTALETF v2 (NG)

pTALETF v2 (NI)pTALETF v2 (HD)pTALEN v2 (NI)pTALEN v2 (NG)

pTALEN v2 (NN)pTALEN v2 (HD)

 

 

 

 

腺病毒系统:

Stratagene3500

VectorspShuttle-CMVpShuttlepAdTrack-CMVpAdTrackpAdEasy-1

StrainsBJ5183AdEasy-1

Invitrogen1000

VectorpAd/BLOCK-iT-DEST RNAi Gateway Vector

Microbix1600

VectorpDC315pBHGloxdelE13cre

腺相关病毒系统:

Stratagene3000

VectorpAAV-MCSpAAV-RCpHelperpAAV-LacZpAAV-IRES-hrGFP

pCMV-MCS

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

逆病毒系统:

RNAi

Clontech1800

VectorsRNAi-Ready pSIREN-RetroQ

RNAi-Ready pSIREN-RetroQ-DsRed-Express

RNAi-Ready pSIREN-RetroQ-ZsGreenLuciferase shRNA Annealed Oligonucleotide

Ambion1000 x2

VectorspSilencer5.1-U6-RetropSilencer5.1-H1-Retro

基因表达载体

Clontech800x2+800x3

VectorspRetroX-IRES-ZsGreen1pRetroX-IRES-DsRed Express

pLNCX2pQCXIHpMSCVpuro

Addgene800 x2+800

VectorspSuper retro puro pSuper retro GFP-neopBabe puro

逆病毒包装载体

Addgene1000

VectorspCMV-Gag-polpCMV-VSV-G

Imgenex1000

VectorspCL Ampho

Stratagene1000

VectorspVPack-GPpCMV VSV G

 

 

 

 

 

 

 

 

 

 

慢病毒系统:

RNAi载体

Addgene800+800+800 x5

VectorspSicoRpSicoR pGK puropLentilox 3.7pLKO.1 puropLKO.3G

         pPRIME-TET-GFP-FF3pPRIME-TREX-GFP-FF3

Clontech1500

VectorspLVX-shRNA1pLVX-shRNA2

SBI1000

VectorspSIH1-H1-CoGFP

Open Biosystems800x2+800x2

VectorspGIPZ emptypGIPZ controlpTRIPZ emptypTRIPZ control

基因表达载体

Clontech

VectorspLVX-DsRed-Monomer-N11000pLVX-AcGFP1-N11000

pLVX-IRES-ZsGreen11000),pLVX-IRES-neo1000pLVX-IRES-td Tomato1000pLVX-IRES-puro1000pLVX-puro1000

pLVX-Tight-PuropLVX-Tet-On-Advanced(一套2000

pLVX-TRE 3GpLVX-Tet 3G(一套2400

Invitrogen1000 x3

VectorpLenti4 TO/V5 DESTpLenti6 V5 DESTpLenti6-UbC-V5-DEST

SBI1000 x2

VectorspCDH-CMV-MCS-EF1-copGFPpCDH-CMV-MCS-EF1-GFP+Puro (pCD513B-1)

Addgene800x3

VectorpWPXLFUGWpLVTH

慢病毒包装载体

Invitrogen1000x3+800x2

VectorspLp1pLp2pLp VSV G

StrainStbl3Stbl4

Cell biolabs800

VectorspCgpV

Clontech800

VectorpVSV G

Addgene

Second generation1000

VectorspCMV-dR8.91pCMV-VSV-G

Second generation1000

VectorspMD2.GpSPAX2

Third generation1200

VectorspMDLg pRREpRSV-revpCMV-VSV-G

iPS慢病毒载体

Cell biolabs1000

VectorspLentG-KOSM

Addgene1000

VectorsFUW-M2rtTATetO-FUW-OSKM

 

SBI (FIV):一套1800

VectorspCDF1-MCS2-EF1-copGFPpFP93

 

建永生化细胞系慢病毒载体

Addgene1000 x3

VectorpLOX-CWBmi1pLOX-TERT-iresTKpLOX-Ttag-iresTK

 

PiggyBac转座子系统

VectorsHA-mPB1000),PiggyBac 5’LTR (pMD18T vector),(800

PiggyBac 3’LTR (pMD18T vector)800

 

SBI:一套2400

VectorsPiggyBac Dual promoter (PB513B-1)Super PiggyBac Transposase (PB200PA-1)

 

信号通路报告系统:

Clontech800/

VectorspGAS-TA-LucpSTAT3-TA-LucpISRE-TA-LucpTA-Luc

pIκB-EGFPpNFAT-TA-LucpCaspase3-sensorpAP1(PMA)-TA-Luc

pCRE-LucpGRE-LucpHSE-Luc

Stratagene 800/

Vectorsp53-LucpAP-1-LucpNF-κB-LucpSRE-Luc

Promega 800/

VectorspGL4.10pGL4.26pGL4.29pGL4.30pGL4.75pGL4.19

Millipore 800/

VectorsTOPFlashFOPFlashSuperTopFlash

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

RNAi系统:

Ambion800+800x6

VectorspSilencer1.0pSilencer 2.1-U6 hygropSilencer 3.1-H1 hygropSilencer 3.1-H1 neo

pSilencer 4.1-CMV neopSilencer 4.1-CMV puro

Reporter plasmidpMIR-REPORT Luciferase

Promega800+800

Reproter plasmidpsiCHECK-2pmir GLO

Oligoengine800

VectorpSuper-puro

Other800

VectorpGenesil-1

Clontech1000

VectorpmR mCherry

SBI1000

VectorpmiRZip anti-microRNA

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

酵母表达系统:

酿酒酵母

Invitrogen1800

VectorspYES2pYES6/CTpYES2/CT/α-factor

StrainINVSc1

毕赤酵母

Invitrogen800/

VectorspGAPZα ApPICZα ApPICZα BpPICZα CpPIC9KpPIC3.5KpPICZ ApPICZ BpPICZ C

StrainsX33KM71KM71HGS115SMD1163SMD1168

Invitrogen2400

VectorpPink HCpPink LC

StrainPichiapink-1Pichiapink-4

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Gateway系统:

Invitrogen800       /

VectorspENTR 1ApENTR3CpDONR221pcDNA6.2-GWEmGFP-miR negative

pDONR Zeo

Regulatory plasmidpLenti6/TR

StrainDB3.1

 

表面展示系统:

酵母表面展示

Invitrogen1000

VectorpYD1

StrainEBY100

哺乳动物表面展示

Invitrogen800

VectorpDisplay

噬菌体表面展示800/

VectorpCantab 5e

StrainHB2151TG1

 

重组蛋白乳汁表达系统:

Invitrogen1000

VectorpBC1

 

绝缘子检测系统:

Invivogen1000

VectorpWHERE

 

果蝇细胞表达系统:

Invitrogen1200

VectorpAc5.1 V5-His BpCo hygro

哺乳动物表达系统

Invitrogen

VectorspcDNA3.1(neo)+ 800pcDNA3.1(neo)- 800pcDNA3.1(hyg)+ 800pcDNA3.1(zeo)+ 800pcDNA3.1/GS800pcDNA3.1(-)/myc-His  A800pcDNA4/HisMax B800pcDNA6-Myc/His B800pSecTag2 A800pVAX1800pBudCE4.1800pTracer CMV2800 pCEP4800pUB6/V5 His A800pUB6/V5 His B800pUB6/V5 His C800pUB6/V5 His lacZ800

pSFV1800pEF1/myc-His B800 pcDNA3.1(+)/Myc HisB800pEF6/Myc-HisC800

Stratagen

VectorpCMV-Tag 2 B800pCMV-Tag 5B800pNTAP-B800pBK-CMV800pIRES-hrGFP-1a800pSG5800

Promega:800 x3

VectorspCI-neopCIpSI

Clontech  800一个

Vectors pCMV-mycpCMV-HApIRESpIRES neo

pIRES hyg3pIRES neo3pIRES puro3EcoRI-NotI之间连入Mus musculus CD1d1 antigen),pIRES2-EGFPpIRES puro2

Sigma

VectorspFLAG CMV2800

Invivogen

VectorspVitro2-neo-mcs800

Addgene

VectorspCAGGS800

 

 

 

 

 

 

昆虫细胞表达系统:

Invitrogen800 x12

VectorspFastBac1pFastBac1-GuspFastBacHT ApFastBacHT BpFastBacHT CpFastBacHT-CATpFastBacDualpBlueBacHis2 ApBlueBacHis2 B

pBlueBacHis2 CpVL1392pVL1393

StrainDH10Bac800

VectorspIZT/V5-His (800)pMIB v5-His B (800)

Cellsf9High Five

 

MultiBac Expression System3000

VectorspFBDMpUCDMpBADZ-His6Cre

StrainsDH10MultiBacBW23473

Cell

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

荧光蛋白报告系统:

Clontech  800/

Vectors pDsRed2-N1pDsRed2-C1 pDsRED-Monomer-N1pmCherry-N1pmCherry-C1, pEYFP-NpEYFP-C1pEGFP-N1pEGFP-C1pEGFP-N3pEGFP-1pAcGFP1-1pECFP-N1pECFP-C1pDsRED2-ERpDsRED2-MitopDsRED2-NucpDsRED2-PeroxipEYFP-GolgipEYFP-MempEYFP-ERpEYFP actinpEYFP tubpEGFP-ActinpECFP-ERpECFP-MitopLEGFP-N1pLEGFP-C1 pDsRed2-1pEGFP-C3pPAmCherry-TubulinpZsYellow-N1pAmCyan-N1

FRET assay800 x2

VectorsDkeima fluorescent proteinmAmetrine

启动子增强子报告系统:

Promega800 x7

VectorspGL3-basicpGL3-controlpGL3-enhancerpGL3-promoter

pRL-TKpRL-CMVpRL-SV40

NEB800

VectorspGluc-basic

 

 

 

 

 

 

 

 

 

 

原核表达系统:

Novagen  800/

VectorspET-11a(+)pET-17b(+)pET-19b(+)pET-20b(+)pET-21a(+)pET-22b(+)pET-26b(+)pET-27b(+)pET-28a(+)pET-28b(+)pET-29a(+)pET-30a(+)

pET-32a(+)pET-39b(+)pET-40b(+)pET-42a(+) pET49b(+)pET50b(+)pETduet-1pACYCDuet-1pRSFDuet-1pCDFDuet-1

StrainsBL21(50)BL21(DE3) BL21(DE3)plysSRosetta(DE3) Rosetta-gami 2(DE3)pLysSRosetta-gami B(DE3)pLysSBLR(DE3) BL21(DE3)goldRosetta Blue(DE3) Rosetta(DE3)plysSRosetta-gami(DE3) Origami(DE3) OrigamiB(DE3)

 

Takara1000

VectorspCold IpCold TF

NEB1500

VectorspMAL-c2xpMAL-c4xpMAL-p4x

Biosee800/

VectorpLLP-OmpApLLP-STIIpMBP-PpMBP-CpET-GSTpET-Trx

pET-HispTrc-CKSpET-DsbA

Stratagene800

StrainBL21 codonplusDE3RIPL

Invitrogen 1000

VectorpRSET A

StrainBL21(AI)

Qiagen1000

VectorpQE30

StrainM15

NEB800x5

VectorspTWIN1pTYB11pMXB10pTXB1

StrainER2566

Amersham800/

VectorspGEX-4T-1pGEX-5X-2pGEX-6P-1pGEX-2TK

Invitrogen800/

VectorspBADHis ApBADHis BpBADHis C

Lucigen800

StrainC43(DE3)

Other1000

VectorpBV220pBluescript II SK(+)

Takara

分子伴侣系统:800/

VectorpG-KJE8pGro7pKJE7pG-Tf2pTf16

 

基因载体:

Invitrogen

VectorpOG44800

Invivogen

VectorspORF-lacZ800pORF-HSV1tk800

 

克隆载体

Promega800/

VectorspUC18pUC19pBR322pSP64pSP73

 

柯斯载体

Stratagene800

VectorSuperCos1

 

同源重组载体1000

VectorpSIM5pSIM6

 

细菌基因敲除系统800/

VectorpKD3pKD4pKD20pKD46PCP2030),

StrainBW25113

 

克隆菌株

StrainsDH5α(800)JM110(800)TOP10(800)XL2-Blue MRF’ (800)Mach1 T1(800)

OmniMAX2 T1Phage-Resistant Cells(800)XL10 Gold(800)EZ10(800)

DH10B(800)SURE(800)

 

基因

Genes human TERT1000Taq1000Pfu (pET16b(+): NdeI-BamHI 2325bp1200)KOD (pET21b+,1800)T4 DNA ligase (pET15b+优化的T4 DNA连接酶1800)hOct4hSox2hKlf4hC-MycmOct4mSox2mKlf4mC-Mychuman CD40Lhuman DHFRhuman Hspa1ahuman IgG1 Fcmouse GM-CSFmouse IFN-gammamouse IL4mouse IL18Cre

         pET28a-TEVklentaqT vector),Vaccinia viru Topoisomerase IT vector),sfGFPiCreLysozymepPICZα AGST-3Cmutant TaqpET28扩增血液DNA),M-MLVSUMOpET28a),mouse RIpET28T4 PNKpET28

 

 

1000/

慢病毒载体

SBI

pCDH-EF1-MCS-T2A-Puro   

pCDH-EF1-MCS-T2A-Puro

Addgene

pLVTHM   1000

pLOX-CW-CRE  1000

酵母表达载体:

pP-αSUMO3   1000

SUMO protease 2   1000

真核表达载体:

pBI CMV1   1000

BL21gold(DE3) plysS   800

pE-SUMO    800

真核表达乳酸菌:

pMG36e    800

MG1363    800