IL27 / Interleukin-27 Protein (His Tag)_人蛋白和抗原_蛋白质/抗原/多肽_生化试剂库_商城

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IL27 / Interleukin-27 Protein

IL27 / Interleukin-27 Protein Price Inquiry ( Available Sizes )

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IL27 / Interleukin-27蛋白产品信息

别名:	p28, IL30, IL-27, IL-27A, IL27p28, MGC71873, IL27
表达方式:	A DNA sequence encoding the IL27 complex composed of IL27B subunit (NP_005746.2) ( Met 1 - Lys 229 ) and the N-terminally truncated IL27p28 (IL27A) subunit ( NP_663634.2 ) ( Phe 29 - Pro 243 ) linked by an polypeptide linker was fused with the Fc region of human IgG1 at the C-terminus.
种属:	Human
表达宿主:	Human Cells

IL27 / Interleukin-27蛋白质控指标

纯度:	> 92 % as determined by SDS-PAGE	SDS-PAGE: IL27 protein
内毒素:	< 1.0 EU per μg of the protein as determined by the LAL method
稳定性:	Samples are stable for up to twelve months from date of receipt at -70℃
预测N端:	Arg 21
分子量:	The recombinant human IL27 complex ( IL27B & IL27A ) consists of 450 amino acids after removal of the signal peptide and has a calculated molecular mass of 50.4 kDa as estimated in SDS-PAGE under reducing conditions.
缓冲液:	Lyophilized from sterile PBS, 10% glycerol

IL27 / Interleukin-27蛋白使用指南

储存方法:	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL27 / Interleukin-27蛋白相关产品和研究主题

蛋白:

抗体:

IL27 / Interleukin-27蛋白背景综述

IL27A, also referred as the IL12 p35-related protein, p28, is one subunit of a heterodimeric cytokine complex, and associates with another subunit EBI3 (EBV-induced gene 3), an IL12 p40-related protein (IL27B). IL-27 is a cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma / IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1 / TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1 / ITGAL / ERK-dependent pathways. IL-27 also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. IL-27 suppresses the expression of GATA3, the inhibitor TH1 cells development. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity and antiangiogenic activity.