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份 库存999份
| 货号： | DMSSY510 |
| 供应商： | 上海笃玛生物科技有限公司 |
| 数量： | 现货 |
| 保存条件： | 电询 |
| 规格： | 100T |
自噬MDC染色步骤
Labeling of Autophagic Vacuoles with monodansylcadaverine (MDC)The autofluorescent agent MDC (Sigma) was recently introduced as a specific autophagolysosome marker to analyze the autophagic process [33]. U251 cells were treated with Res 150 μM for 3–24 h. Autophagic vacuoles were labeled with MDC by incubating cells with 0.05 mM MDC in RPMI1640 at 37°C for 10 min at various times after Res treatment. After incubation, cells were washed three times with PBS and immediately analyzed with a fluorescence microscope (Nikon Eclipse TE 300, Japan) equipped with a filter system (V-2A excitation filter:380/420 nm, barrier filter:450 nm) [34]. Images were captured with a CCD camera and imported into Adobe Photoshop.
Labeling of Autophagic Vacuoles with monodansylcadaverine (MDC)The autofluorescent agent MDC (Sigma) was recently introduced as a specific autophagolysosome marker to analyze the autophagic process [33]. U251 cells were treated with Res 150 μM for 3–24 h. Autophagic vacuoles were labeled with MDC by incubating cells with 0.05 mM MDC in RPMI1640 at 37°C for 10 min at various times after Res treatment. After incubation, cells were washed three times with PBS and immediately analyzed with a fluorescence microscope (Nikon Eclipse TE 300, Japan) equipped with a filter system (V-2A excitation filter:380/420 nm, barrier filter:450 nm) [34]. Images were captured with a CCD camera and imported into Adobe Photoshop.
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