Catalog Number:
500-P55
Preparation:
Produced from sera of rabbits immunized with highly pure recombinant Murine IL-5. Anti-Murine IL-5 specific antibody was purified by affinity chromatography employing an immobilized Murine IL-5 matrix.
Immunogen:
E.coli derived Recombinant Murine IL-5 (PeproTech catalog #215-15)
Application:
ELISA
SandwichTo detect Murine IL-5 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-5 (500-P55Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5. ELISA: Sandwich
Western Blot
To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Western Transfer (Western Blot)
Research Interests:
| Immune System | | Inflammation |
| Proliferation | | Wound Healing |
| Allergy | |
Country of Origin:
USA