Preparation : | Produced in BALB/c mice using highly pure (>98%) recombinant human VEGF165 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A chromatography. |
Catalog #: | 500-M88 |
Source : | Mice |
Formulation : | A sterile filtered antibody solution was lyophilized from PBS. |
Stability : | The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. Frozen aliquots are stable for at least six weeks at 2-8oC or for at least six months at -20oC. Avoid repeated freeze-thaw cycles. |
Reconstitution: | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml. |
Applications : | |
Selected References : | Selected List of References Citing This PeproTech Product |
Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10.0 ng/ml), a concentration of <0.2 μg/ml="" of="" this="" antibody="" is=""> |
Elisa | In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant human VEGF165 when used with PeproTechs biotinylated antigen affinity purified anti-human VEGF165 (500-P10Bt) as the detection antibody at a concentration of approximately 0.25-1.0 μg/ml. |
Western Blot | To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions. |
Country of Origin : | USA |
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| | Size B | 500μg | $150 |  | | |
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| Special Sizes: Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request. |
| Usage:For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
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[VIP第1年] 指数:1
