Preparation : | Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human TRAIL/APO-II as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein A affinity chromatography. |
Catalog #: | 500-M49 |
Source : | Mice |
Formulation : | A sterile filtered antibody solution was lyophilized from PBS. |
Stability : | The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. Frozen aliquots are stable for at least six weeks at 2-8oC or for at least six months at -20oC. Avoid repeated freeze-thaw cycles. |
Reconstitution: | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml. |
Applications : | |
Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of hTRAIL/Apo2L (10.0 ng/ml), a concentration of 0.30-0.60 μg/ml of this antibody is required. |
Elisa | In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant human TRAIL/APO-II when used with PeproTechs biotinylated antigen affinity purified anti-human TRAIL/APO-II (500-P135Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml. |
Western Blot | To detect hTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions. |
Country of Origin : | USA |
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| | Size B | 500μg | $150 |  | | |
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| Special Sizes: Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request. |
| Usage:For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
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[VIP第1年] 指数:1
