ibidi 培养&染色 u-Slide I/

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 A combination of a cell culture chamber and a coverslip for imaging inside a channel---ibidi

 

·                                 Large observation area for microscopy

·                                 Small volume in channel

·                                 Long-term cultivation possible

 

Applications:

·                                 Immunofluorescence stainings with easy liquid exchange

·                                 Real-time imaging during staining process

·                                 High-resolution microscopy of living and fixed cells

 

Specifications:

 

Recommended volume per reservoir

600 μl

Number of channels

1

Volume of the channel

100 μl

Height of the channel

0.4 mm

Length of the channel

50 mm

Width of the channel

5 mm

Growth area

2.5 cm2

Bottom: ibidi Standard Bottom

 

 

 

Technical Features:

·                                 Lids for long-term cultivation

·                                 See also μ-Slide I Luer 

 

Example: Seeding Cells into a μ-Slide I
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Step 1:   Fill the channel with 100 μl cell suspension
Step 2:Fill one reservoir with cell-free medium
Step 3:Fill second reservoir with the same amount of cell-free medium to equilibrate the system