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μ-Slide 4 well Ph+/80446
μ培养载玻片 4孔Ph+
Excellent Phase Contrast in the μ-Slide 4 well Ph+
一个开放的四孔培养载玻片，带有一个特殊中间板，非常适用于相差和高端的荧光显微镜
整个培养孔具有优秀的相差-没有弯月面现象
体化小室，既可用于细胞培养，也可用于显微镜成像
使用高分辨率显微镜可以通过玻璃片底部进行样品观察
没有盖玻片，不会泄露，用于快速，简单的免疫荧光。
| 货号 | 产品名称 | 规格（个/盒） |
| 80446 | μ-Slide4孔Ph+腔室载玻片，ibiTreat底部处理 | 15 |
| 80442 | μ-Slide 4孔Ph+腔室载玻片，Collagen IV底部处理 | 15 |
| 80443 | μ-Slide 4孔Ph+腔室载玻片，Fibronectin底部处理 | 15 |
| 80444 | μ-Slide 4孔Ph+腔室载玻片，Poly-L-Lysine底部处理 | 15 |
| 80445 | μ-Slide 4孔Ph+腔室载玻片，Poly-D-Lysine底部处理 | 15 |
| 80441 | μ-Slide 4孔Ph+腔室载玻片，无包被 | 15 |
| 80447 | μ-Slide 4孔Ph+腔室载玻片，玻璃底 | 15 |
应用
细胞培养以及细胞培养物的显微镜观察。
转染分析
活细胞和固定细胞的免疫荧光显微镜观察
长时间段下活细胞成像
技术指标
| 孔数量 | 4 | 带盖子高度 | 10.8 mm |
| 孔尺寸大小 | 21.2 x 11.0 x 3.0 | 每孔生成面积 | 2.2 cm2 |
| 每孔体积 | 700 μl | 每孔生成面积 | 5.9 cm2 |
| 底部ibidi 标准底部 | |||
技术特点
开放式，两个独立的培养孔载玻片
每个培养孔上的中间板可以避免弯液面形成
从两侧缝隙很容易加液，无气泡形成，
适用于显微镜的光学成像特质
兼容染色，固定
细胞粘附的表面Ibitreat
生物兼容性塑料生产，无胶水，不泄露
Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.
μ-Slide 4 well μ-Slide 4 well Ph+
Working with the μ-Slide 4 well Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.
| Cross Section Through a Well of the μ-Slide 4 well Ph+ with a Transmitted Light Beam Path
| |
The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.
Working with the μ-Slide 4 well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.
Comparison Ph+ Well versus Standard Well: No Meniscus Effect in Ph+ Well
μ-Slide 4 well Ph+Excellent Phase ContrastExcellent Fluorescence Microscopy
| Standard Well Poor Phase Contrast Excellent Fluorescence Microscopy |
[VIP第1年] 指数:1
