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制造商（Manufacturer）：日本ECI株式会社（ECI Inc., Japan ）主要技术指标（Main technical indicators）： 物镜：10×20×40×100×（Objective lens: 10×20×40×100×）荧光滤块：B/G/R (Fluorescent filter block: B/G/R)样品量：≤100个细胞(Sample amount: 100 or less cells)温度控制：室温+ 3℃～40℃（Holder temperature control: room temperature+ 3℃～40℃）硅基底芯片:通道深度4μm,5μm ,6μm,8μm(Chip terrace depth：4, 5, 6, or 8μm)
12个独立通道，可同时进行12例试验(12 channels, up to 12 concurrent assays)自动聚焦系统(Autofocus system)
制造商（Manufacturer）：日本ECI株式会社（ECI Inc., Japan ）主要技术指标（Main technical indicators）： 物镜：10×20×40×100×（Objective lens: 10×20×40×100×）荧光滤块：B/G/R (Fluorescent filter block: B/G/R)样品量：≤100个细胞(Sample amount: 100 or less cells)温度控制：室温+ 3℃～40℃（Holder temperature control: room temperature+ 3℃～40℃）硅基底芯片:通道深度4μm,5μm ,6μm,8μm(Chip terrace depth：4, 5, 6, or 8μm)12个独立通道，可同时进行12例试验(12 channels, up to 12 concurrent assays)自动聚焦系统(Autofocus system)动态影像实时记录 (Data store as movie image file)计算机分析系统，包含浓度梯度的精确测量，自动统计细胞数量，细胞形态变化、迁移速度、迁移方向等统计学分析。主要功能（Main function）：硅基底芯片，其上嵌刻的水平通道可形成化学趋化因子浓度梯度，用于测定浓度梯度依赖细胞的功能，如趋化，脱颗粒。水平通道的深度小于悬浮细胞的直径，其内可观测细胞形态学变化和增殖迁移过程。细胞趋化分析不仅包括中性粒细胞、嗜酸性粒细胞、单核细胞、淋巴细胞等外周血白细胞，也包括各种癌细胞和培养细胞，如平滑肌细胞、内皮细胞、神经细胞、干细胞等。还可分析蛋白质及细胞相互作用、细胞信号转导、细胞骨架、钙流入、活性氧代谢等。可应用于趋化因子及药物筛选、炎症、过敏反应、肿瘤、神经、免疫、心血管、干细胞等方面的研究。
日本ECI株式会社细胞动态可视化系统设备TAXIScan-FL，是全新光学动态成像与活体细胞处理技术的完美结合，本设备采用专利TAXIScan技术，具有独立知识产权，其核心部件为硅基底芯片，其上嵌刻的水平通道可形成化学趋化因子浓度梯度；水平通道的深度精度小于悬浮细胞的直径，可精确到微米级别，其内可观测细胞形态学变化和增值迁移过程；成像部件冷光CCD相机定位于观测平面以下，配有高性能透镜和同轴反照明装置；基于以上的革命性技术使实验只需100个甚至更少的细胞样本；根据实验具体要求自定义设置实验条件参数。
Fluorescent filter block capacity | Up to 5 blocks |
Applicable fluorescent molecules | Most commercial fluorphores |
Bright field optical system | Reflection plane image system |
Objective lens | 10X 20X 40X and 100X |
Focus system | Autofocus compatible |
Number of channels | 12 |
Holder temperature control | Room temperature + 3℃~40℃ |
Light source | Mercury lamp |
Camera | Monochrome cooled CCD camera |
Equipment control and data analyzing software | NIS-elements (manufactured by Nikon ) |
Main body dimension | Width 900mm X depth 750mm X height(excluding monitor)1100mm |
Rated voltage | AC 100V 50/60Hz |
Wattage | 600VA |
 PRODUCTS |
TAXIScan is ECIs unique technology to form chemoattractant concentration gradient in a small space so as to observe cellular chemotaxis movement with good repeatability. Existing model of EZ-TAXIScan with a mere 10X objective lens, limited conventional bright-field observation and up to 6 sample simultaneous assays is appreciated well for its easy use of TAXIScan functions.Newly introduced TAXIScan-FL makes fluorescent observation possible. The equipment is mounted with up to 40X objective lens and bright field observation system with the use of differential interferometry. The number of simultaneous assay samples is increased up to 12 so that higher analysis with enhanced throughput can be anticipated.
[NEW]Chemotactic Cell Movies are now available | |
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| Click the pictureto see chemotactic cell movie |
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- The equipment enables fluorescent observation for various types of cell performing chemotaxis movement. Mounted with high-power objective lens (up to 40X）and differential interferometry optical system enable clear image acquisition at bright field observation. The employment of autofocus mechanism also enables stable focus for extended period to time.
- Simultaneous observation of transient increase (in fluorescent strength) of intracellular calcium concentration and chemotaxis movement were performed using cells stained with fluorescent calcium indicator (Fluo-3) (cell: human monocyte, chemoattractant: hMIP-1a)
- EZ-TAXIScan made observation of degranulation phenomena in mast cell possible. In contrast, TAXIScan-FL enables bright field observation in much higher magnification and differencial interferometry. By doing fluorescent straining for mast cells (blue : nucleus, green : calcium concentration, orange : degranulation), it becomes possible to observe transient calcium concentration increase in each cell before degranulation, etc. more in detail.
- Measurement record
- Main specification
