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Disease: Burkitts lymphoma Origin: Established from the left maxilla of a 12-year-old African boy with Burkitt lymphoma in 1963; first continuous human hematopoietic cell line Species: Homo sapiens Tissue: Lymphoblast Morphology: Lymphoblast Properties: Round single cells in suspension Cytogenic data: human flat-moded hypotetraploid karyotype with 12% polyploidy - 89(80-91)<4n>XXYY, -4, -9, -12, -16, -18, -21, +3mar, add(4)(q35), add(6)(q27)x2, t(8;14)(q24;q32), der(8)t(8;14) (q24;q32)add(8)(?p12), del(9)(p23), der(14)t(8;14)(q24;q32) - carries t(8;14) associated with Burkitt lymphoma - closely resembles depositors karyotype Patient: Male, Black, 11 yrs of age Medium: RPMI1640 + 10% FBS Subculture: Optimal split ratio of saturated culture at 1:4 to 1:8 twice a week; seed out at 0.5 x 10^6 cells/ml; maximum of 1.5x10^6 viable cells/mL is considered saturated culture; 5% CO2; 37 °C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSOBiosafety Level: I (The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1). Sterility:Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X,Y; CSF1PO: 10,12; D13S317: 13; D16S539: 8,11; D5S818: 10,13; D7S820: 10; THO1: 6,7; TPOX: 8,13; vWA: 16,19 References for Raji cells:1. Pulvertaft JV. Cytology of Burkitts tumour (African lymphoma). Lancet 1: 238-240, 1964.
[VIP第1年] 指数:1
