点击图片查看原图
Disease:Burkitts lymphoma Origin: Established from the tumor mass of an African child with Burkitt lymphoma in 1967; cells express MYC mRNASpecies: Homo sapiens Tissue:B lymphocyte Morphology:Lymphoblast; lymphoblastoid Properties: Single cells or small clusters in suspension Cytogenic data: Human hypodiploid karyotype with 4% polyploidy - 44(43-46)<2n>X, -Y, -5, -6, +7, -10, -13, -21, +3mar, ins(1;?)(p11;?), ins(3)(q11q29), der(5)t(1;5)(q25;q35), del(8)(p22), der(14)t(14;?)(p11;?), der(15)t(15;21)(p11;q11) - a supernumerary ring or fragment present in 8% of cells - NAMALWA marker chromosomes present Patient: Female, African, 3 yrs of age Medium: RPMI-1640 Medium + 10% FBS + 10 mM HEPES + 1.0 mM sodium pyruvate Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Maintain at 0.5-1.5 x 10^6 cells/ml; split 1:2 to 1:3 every 2-3 days; subculture cells at 1 x 10^6 cells/ml; 5% CO2; 37°C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSOBiosafety Level:II [ Cells Contain Herpesvirus (EBV) and Squirrel monkey retrovirus (SMRV)] Sterility:Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X; CSF1PO: 10,11; D13S317: 11,12; D16S539: 9; D5S818: 12,13; D7S820: 11; THO1: 7,9.3; TPOX: 6,11; vWA: 14 References for NAMALWA cells:1. Nadkarni, J. S., Nadkarni, J. J., Clifford, P., Manolov, G., Fenyo, E. M., Klein, E. (1969). Characteristics of new cell lines derived from Burkitt lymphomas. Cancer 23 ( 1 ): 64-79. images/sell/4178827 2. Klein F, et al. Large-scale production and concentration of human lymphoid interferon. Antimicrob. Agents Chemother. 15: 420-427, 1979. PubMed: 464569 images/sell/464569
[VIP第1年] 指数:1
