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| 货号： | ASK-3006 |
| 保存条件： | -20度 |
| 数量： | 大量 |
| 英文名： | Human ES/iPS Cell Characterization Kit |
| 供应商： | Applied stem cell |
| 规格： | 20 次反应 |
ASC’s human embryonic stem/induced pluripotent stem (hES/iPS) cell Characterization Kit is designed for users who need to examine the pluripotent status of hES/iPS cell by analyzing undifferentiated protein marker expressions. Incorporated a standard cell culture and immunofluorescence staining protocol with our optimized, ready-to-use reagents, the kit provides a fast, convenient, sensitive immunodetection for status ofhES/iPS cell. Five widely adopted antibody markers, anti-Oct4, anti-Sox2, anti-SSEA-3, anti-TRA-1-60 and anti-TRA-1-81, are offered here with optimized concentration. Oct4 and Sox2 are transcription factors highly expressed in undifferentiated ESC and embryonic germ cells (EGC)[1-3]. SSEA-3 is a globoseries carbohydrate antigen present on the surface of human ESC, but not mouse ESC[4,5]. TRA-1-60 and TRA-1-81 antigens are expressed on the surface of human teratocarcinoma stem cells (EC), hEGC and hESC[6], but no expression in murine EC, EG or ES cells. In addition, ASC’s hES/iPS cell Characterization Kit also provides a rapidalkaline phosphatase(AP)activity test, which is widely used to examine the differentiated/undifferentiated status of ES/iPS cells. AP is a stem cell membrane marker. Elevated expression of AP is associated with pluripotent status[7].
Contents and Storage
All regents supplied here are sufficient for 20 reactions according to the protocol below and also available from Applied StemCell Inc. separately.
| Material | Amount | Concentration | Catalog No. | Storage & Stability |
| Anti-Oct4 (h) | 800 µL | 1´ | ASA-0110 | Store at +4˚C for short term (up to 2 weeks). For long term storage, aliquot and store at -20˚C to -80˚C. Avoid repeat freeze/thaw cycles. |
| Anti-Sox2 (h) | 800 µL | 1´ | ASA-0120 | |
| Anti-SSEA-4 | 800 µL | 1´ | ASA-0150 | |
| Anti-TRA-1-60 | 800 µL | 1´ | ASA-0160 | |
| Anti-TRA-1-81 | 800 µL | 1´ | ASA-0170 | |
| Fixation solution* | 15 mL | 1´ | ASB-0101 | |
| Goat-anti-mouse * | 3 mL | 1´ | ASA-0001 ** | 4˚C, in dark |
| Goat-anti-rabbit * | 2 mL | 1´ | ASA-0003 ** | |
| DNA staining solution * | 5 mL | 1´ | ASB-0104 | |
| Permsolution | 15 mL | 1´ | ASB-0102 | 4˚C |
| Blocking solution | 15 mL | 1´ | ASB-0103 | 4˚C |
| Mounting solution * | 4 mL | 1´ | ASB-0105 | 4˚C, in dark |
| AP test solution * | 1.5 mL | 1´ | ASB-0001 | 4˚C, in dark |
**ASA-0001 and ASA-0003 is superior red-fluorescent Alexa Fluor 594 dye based 2nd antibody which gives off a red fluorescence with wavelength at 617 nm. But we also provide other color 2nd antibody upon customer’s request.
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