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份 库存999份
| 货号： | R0176 |
| 保存条件： | -20 |
| 规格： | V S L |
DpnI R0176L S V
| R0176L | DpnI | 5,000 units |
| R0176S | DpnI | 1,000 units |
| R0176V | DpnI | 500 units |
Description
Product Source
An E. coli strain that carries the DpnI gene from Diplococcus pneumoniae G41 (S. Lacks).Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| CutSmart™ Buffer | -20 | 10X |
Properties and Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA (dam methylated) in 1 hour at 37°C in a total reaction volume of 50 µl.Reaction Conditions
1X CutSmart™ Buffer
Incubate at 37°C
1X CutSmart™ Buffer:
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 μg/ml BSA
pH 7.9 @ 25°C
Activity in NEBuffers
NEBuffer 1.1: 100%NEBuffer 2.1: 100%
NEBuffer 3.1: 75%
CutSmart™ Buffer: 100%
Diluent Compatibility
- Diluent B
Storage Temperature
-20°CStorage Conditions
10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
80°C for 20 minMethylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked by Overlapping
Notes
- DpnI cleaves only when its recognition site is methylated. DNA purified from a dam+ strain will be a substrate for DpnI.
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