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份 库存999份
| 货号： | FXP001 |
| 数量： | 大量 |
| 英文名： | RBC Lysing Buffer (10x) |
| 规格： | 100ml/200ml/500ml/50 Tests/100 Tests/500 Tests |
Catalog No. Size
FXP001-1000 1000 Tests
FXP001-500 500 Tests
FXP001-100 100 Tests
FXP001-050 50 Tests
Storage Condition: Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
Applications: FC
Description:
Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single
cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing
ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. This
buffer can be used for lysis of human, mouse, rat, rabbit and non-human primate samples.
Preparation:
This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. The
pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to
room temperature prior to use.
Lysis of Human Peripheral Blood RBCs:
1. Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to
room temperature prior to use.
2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 µl of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected
from light, for 10-15 minutes.
4. Centrifuge 1000rpm for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer (e.g., 4abio Cell Staining Buffer, Cat. No.FXP005), wash 1X.
6. Resuspend and proceed with further procedures.
温馨提示：不可用于临床治疗。
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