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份 库存999份
| 货号： | A-P-2027 |
| 保存条件： | 常温+低温 |
| 供应商： | A&D Technology |
| 数量： | 10 |
| 英文名： | ChromaFlash High Sensitivity ChIP Kit |
| 保质期： | 1年 |
| 规格： | 24次、48次 |
高敏免疫沉淀试剂盒 货号：A-P-2027
ChromaFlash High Sensitivity ChIP Kit
背景资料：Protein-DNA interaction plays a critical role for cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanisms of protein-DNA interaction is important for understanding cellular processes. Chromatin immunoprecipitation (ChIP) offers an advantageous tool for studying such protein-DNA interactions. It allows for the detection of a specific protein bound to a specific gene sequence in living cells using PCR (ChIP-PCR), microarrays (ChIP-chip), or sequencing (ChIP-seq). For example, measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while the recruitment of methylated H3-K9 to the promoters on a genome-wide scale can be detected by ChIP-on-chip or ChIP-sequencing. ChIP analysis requires that ChIPed DNA contains minimal background in order to reliably identify true TF-enriched regions. High background in ChIP is mainly caused ineffective wash buffers, insufficient cross-link reversal, inappropriate DNA fragment length, and residual RNA interference. To effectively capture TF/DNA complexes, which are often in low abundance, an ideal ChIP method requires having maximum sensitivity with minimized background levels. This method should also be able to enrich highly abundant protein/DNA complexes using a small amount of cells or tissues in a high throughput format. Epigentek’s ChromaFlash™ High-Sensitivity ChIP Kit is designed to achieve these goals by maximizing sensitivity and minimizing non-specific background signals.
产品描述：This kit includes a positive control antibody (RNA polymerase II), a negative control non-immune IgG, and GAPDH primers that can be used as a positive control to demonstrate the efficacy of the kit reagents and protocol. RNA polymerase II is considered to be enriched in the GAPDH gene promoter that is expected to be undergoing transcription in most growing mammalian cells and can be immunoprecipitated by a RNA polymerase II antibody but not by non-immune IgG. Immunoprecipitated DNA is then cleaned, released, and eluted. Eluted DNA can be used for various downstream applications such as ChIP-PCR, ChIP-on-chip, and ChIP-seq。
产品特点：
1·The highly efficient enrichment ratio of positive to negative control is > 500. An extremely low number of cells (as low as 2,000 cells per ChIP reaction) can be used for enriching highly abundant protein/DNA complexes；
2·Optimized buffers and protocol allow minimal ChIP background by overcoming the weaknesses that cause non-specific enrichment, thereby increasing sensitivity and specificity of the ChIP reaction；
3·Increased antibody selectivity and capture efficiency through the use of unique chimeric proteins containing the maximum number of IgG binding domains coated on the strip-wells. This allows strong binding of any IgG subtype antibodies within a wide pH range regardless if they are in monoclonal or polyclonal form；
4.Fast 5 hour procedure, from input chromatin to ready-to-use DNA eluate；
5.96-well plate format makes the assay flexible. Either (a) manual with one single reaction each time; or (b) high throughput with 24-48 reactions each time；
6.Wide downstream analysis compatibility. Compatible with various downstream analysis workflows including ChIP-PCR, ChIP-on-chip, and ChIP-seq。
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 | 规格 | 操作手册 | 货号 | 询价 |
| 高敏免疫沉淀试剂盒 | 24 次 | A-P-2027-24 | ||
| 高敏免疫沉淀试剂盒 | 48 次 | A-P-2027-48 | ||
| 高敏免疫测序试剂盒 | 12 次 | A-P-2030-12 | ||
| 高敏免疫测序试剂盒 | 24 次 | A-P-2030-24 | ||
| RNA甲基化修饰试剂盒 | 50 次 | A-P-9003-050 | ||
| RNA甲基化修饰试剂盒 | 200 次 | A-P-9003-200 | ||
| 亚硫酸盐快速DNA文库制备试剂盒(illumina) | 12 次 | A-P-1055-12 | ||
| 亚硫酸盐快速DNA文库制备试剂盒(illumina) | 24 次 | A-P-1055-24 | ||
| 高敏感亚硫酸盐测序试剂盒(illumina) | 12 次 | A-P-1056-12 | ||
| 高敏感亚硫酸盐测序试剂盒(illumina) | 24 次 | A-P-1056-24 | ||
| SETDB1特异基因敲除定量试剂盒-表观遗传调控因子 | 96 次 | A-P-5002-SETDB1-96 | ||
| 通用乙酰化组蛋白H3K36定量分析试剂盒(荧光法) | 96 次 | A-P-4019-96 |
表观遗传学产品全面解决方案列下：
| 产品名称 | 规格 | 操作手册 | 货号 | 询价 |
| DNA羟甲基化定量试剂盒(荧光法) | 48 次 | A-P-1037-48 | ||
| DNA羟甲基化定量试剂盒(荧光法) | 96 次 | A-P-1037-96 | ||
| DNA羟甲基化定量试剂盒(比色法) | 48 次 | A-P-1036-48 | ||
| DNA羟甲基化定量试剂盒(比色法) | 96 次 | A-P-1036-96 | ||
| DNA甲基化定量试剂盒(荧光法) | 48 次 | A-P-1035-48 | ||
| DNA甲基化定量试剂盒(荧光法) | 96 次 | A-P-1035-96 | ||
| DNA甲基化定量试剂盒(比色法) | 48 次 | A-P-1034-48 | ||
| DNA甲基化定量试剂盒(比色法) | 96 次 | A-P-1034-96 | ||
| 通用DNA甲基化试剂盒(定性分析) | 48 次 | A-P-1011-48 | ||
| 通用DNA甲基化试剂盒(定性分析) | 96 次 | A-P-1011-96 | ||
| 超敏DNA甲基化定量试剂盒 | 48 次 | A-P-1021-48 | ||
| 超敏DNA甲基化定量试剂盒 | 96 次 | A-P-1021-96 | ||
| 总DNA甲基化定量试剂盒 | 48 次 | A-P-1014-48 | ||
| 总DNA甲基化定量试剂盒 | 96 次 | A-P-1014-96 |
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5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
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时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。
