凝胶回收及PCR产物纯化试剂盒_总RNA分离纯化_核酸纯化_生化试剂库_商城

货号：	740609.250
保存条件：	常温
规格：	250次

公司现经销德国MN品牌凝胶回收和PCR产物纯化试剂盒，欢迎大家订购！

产品详细描述

PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume

• Two applications in one kit, one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume: 15 μL – highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)


Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	< 400 µL PCR reaction mixture < 400 mg TAE / TBE agarose gel
Fragment size	50 bp–approx. 20 kbp
Typical recovery	70–95%
A260/A280	1.8–1.9
Elution volume	15–30 µL
Preparation time	10 min/6 preps
Binding capacity	25 µg

Applications

• Purification of PCR products • Extraction of DNA from agarose gels
• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as: - nucleotides, primers - enzymes - mineral oil - PCR additives (e.g., salts, betaine, DMSO) - detergents (e.g., Tween 20, Triton X-100) - dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain)
• Support protocol for DNA extraction from polyacrylamide gels • Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”) • Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the kit, see “Ordering information”)
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis

温馨提示：不可用于临床治疗。

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凝胶回收及PCR产物纯化试剂盒

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