植物微粒体蛋白抽提MM-018

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单价: 5254.00
品牌: Minute
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更新: 2021-07-01
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货号: MM-018
保存条件: -20℃
供应商: Invent Biotechnologies
英文名: Plant Microsomal Membrane Extraction Kit
规格: 50preps

Minute™ Plant Microsomal Membrane Extraction Kit

 


 

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Isolation of microsomal membranes from plant tissues is a common laboratory procedure. Microsomal fraction of plant cell lysate is the focus of interest in many plant research projects. Microsomal fraction is believed to be enriched for plasma membranes, endoplasmic reticulum, Golgi apparatus, vacuolar membranes and other components of membrane system. Traditional method for microsomal fraction isolation involves so called differential pelleting protocol where a series of centrifugation steps are required to obtain various membrane fractions. Traditional protocol for microsomal fraction isolation requires large amount of starting material and employs tedious ultracentrifugation steps. MM-018 offers a simple, rapid and user friendly approach for microsomal membrane extraction using small amount of starting material (200 mg). Water soluble cytosolic proteins are removed during the procedures and water insoluble microsomal fraction, especially plasma membrane fraction, is extracted with optimized buffers in a table-top microcentrfuge. The procedure is simple, rapid and no special instrument required. Native microsomal proteins can be isolated from plant tissue in about one hour without ultracentrifugation. The protein yield is in the range of 100-200 µg/sample.

 

Cat# MM-018  

 

Plant Microsomal Membrane Extraction Kit

 

Kit Components

Item Amount
Buffer A 25 ml
Buffer B 25 ml
Filter Cartridges 50
2.0 ml Collection tubes with cap 50
Plastic rod 4
温馨提示:不可用于临床治疗。