| Zeocin 可选择Sh ble基因表达的细胞。与现在使用的其他动物细胞标记物无交叉抗性。因此这种抗生素可用来分离对其他筛选剂（如：庆大霉素，潮霉素）有抗性的克隆。Zeocin 是一种属于争光霉素家族的糖蛋白抗生素，在体内能作用于大多数细菌（包括E。coli）、真菌（如：酵母菌）、植物细胞、动物细胞、细胞培养级。
使用:
●液体Zeocin 可用于细胞培养。
●用于筛选转化株的 Zeocin 浓度根据pH值和盐浓度改变。
●pH越高，盐浓度越低 Zeocin 活性越大。
●普通大肠杆菌菌株筛选可在25＆micro；g／ml Zeocin 浓度的低盐LB琼脂培养基中进行，pH调至7.5。
●动物细胞筛选：根据不同细胞系从100到750＆micro；g／ml。
●植物细胞筛选：根据不同的细胞种类和培养基从50到250＆micro；g／ml。
●酵母筛选：取决于种属和培养基，S。cerevisiae Sh ble转化株在50＆micro；g／ml浓度的YPD培养基中筛选，pH7.0.304015.
Invitrogen相关资料如下:
Cat. No. R250-01
Zeocin ™ Selection Reagent
Description
Zeocin™ Reagent is a member of the bleomycin antibiotic family. Resistance is conferred by the Sh ble gene which encodes a 13,665 dalton protein. In cells expressing the protein, Zeocin™ is prevented from binding and cleaving the cellular DNA. Zeocin™ is effective on most aerobic cells and can be used for selection in mammalian and insect cell lines, yeast, and bacteria. The amount of Zeocin ™ required for selection will vary between 50-2000 ug/ml (typically 300 ug/ml) depending on the cell type.
C55H83N19O21S2Cu
Formula Weight: 1137.41 g/mole
Zeocin ™ is a trademark of CAYLA.
The ble resistance gene is the subject of one or more of U.S. Patent Nos. 5,021,344 and 5,118,620 and foreign equivalents owned by Cayla and licensed to Invitrogen. This product is sold for research purposes only. For commercial license information, please contact: Licensing Department, Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA 92008.
General Speci fica tions
Concentration: 100 mg⁄mL
Brand: Zeocin ™
Form: Liquid
Product Size: 1 g
Validated Application: Eukaryotic Selection⁄Stable Cell Line Generation
Bacterial Selection
Contents & Storage
Zeocin ™ is provided in solution at a concentration of 100 mg/ml. The 1 gram size is supplied in 8 vials. The 5 gram size is supplied in one vial. Store Zeocin ™ at -20°C in the dark. Zeocin ™ is guaranteed stable for 6 months when properly stored. |
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