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份 库存999份
| 货号： | AE23391PI |
| 样本： | Serum, Plasma, Other biological fluids |
| 标记物： | P12821 |
| 适应物种： | Pig (Sus scrofa; Porcine) |
| 应用： | Quantitative |
| 检测方法： | Sandwich |
| 检测限： | 11.2 pg/mL |
| 供应商： | 华夏远洋 |
| 数量： | 999 |
| 规格： | 96T/48T |
别名：ACE1; CD143; DCP; DCP1; MGC26566; MVCD3; CD143 antigen|angiotensin I converting enzyme 1|angiotensin converting enzyme; somatic isoform|carboxycathepsin|dipeptidyl carboxypeptidase 1|kininase II|pep
检测范围：31.25-2000 pg/mL
产品概述：Angiotensin I-converting enzyme (ACE, EC 3.4.15.1), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), which mediates extracellular volume (i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. ACE Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. The testis-specific isoforn is expressed in spermatocytes, adult testis. ACE can Convert angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin.
特异性：This assay has high sensitivity and excellent specificity for detection of Pig ACE. No significant cross-reactivity or interference between Pig ACE and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Pig ACE and the recovery rates were calculated by comparing the measured value to the expected amount of Pig ACE in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pig ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate ACE in samples. An antibody specific for ACE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACE bound in the initial step. The color development is stopped and the intensity of the color is measured.
温馨提示：不可用于临床治疗。
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