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份 库存999份
| 货号： | GN1176 |
| 供应商： | 上海盖宁生物 |
| 数量： | 现货 |
| 英文名： | pMIB/v5-His B |
| 保质期： | 三年 |
| 保存条件： | 常温 |
| 规格： | 5ug质粒 |
| 质粒类型: | 昆虫细胞表达载体 |
|---|---|
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | OpIE2 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3600bp |
| 5' 测序引物及序列: | OpIE2-F: 5'd[CGCAACGATCTGGTAAACAC]3' |
| 载体标签: | HBM (Nterm), V5 (Cterm), His (Cterm) |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 筛选标记: | Blasticidin |
| 备注: | 在昆虫细胞中能够稳定表达，HBM标签可实现目的蛋白的分泌表达 |
订购信息
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| 1176 | pMIB/v5-His B | 5ug质粒 | ¥1000.00 |
质粒图谱
载体描述
载体描述
pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:
- The OpIE2 promoter for constitutive expression
- The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
- The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
- C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin
温馨提示：不可用于临床治疗。
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